Example sentences for: blotting

How can you use “blotting” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Membranes were washed with TBST and then incubated for 1 hour at room temperature with a secondary antibody followed by washing three times with TBST, immersed in ECL Plus western blotting detection system (Amersham Pharmacia Biotech, Buckinghamshire, England) for 1 min and then exposed to Kodak X-ray film (Rochester, NY).

  • This was accomplished by subjecting cell lysates to electrophoresis in agarose gels, followed by blotting to nitrocellulose membranes.

  • TNF cytotoxicity assay and Western blotting

  • After blotting, the PVDF membrane was stained with 2% amido black in 7% glacial acetic acid and the protein fronts of all lanes were compared for loading accuracy.

  • The urothelium contains a rich store of pre-formed MIF [ 10 11 ] . Moreover, MIF protein and mRNA were also detected by Western blotting techniques and RT-PCR in the major pelvic ganglia (MPG) that provides efferent (parasympathetic and sympathetic) innervation to the bladder and other pelvic viscera [ 12 13 ] . In addition, the L6-S1 dorsal root ganglia (DRG) that provide afferent innervation to the bladder [ 13 ] and other pelvic viscera constitutively expressed MIF.


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