Words similar to blotting
Example sentences for: blotting
How can you use “blotting” in a sentence? Here are some example sentences to help you improve your vocabulary:
Membranes were washed with TBST and then incubated for 1 hour at room temperature with a secondary antibody followed by washing three times with TBST, immersed in ECL Plus western blotting detection system (Amersham Pharmacia Biotech, Buckinghamshire, England) for 1 min and then exposed to Kodak X-ray film (Rochester, NY).
This was accomplished by subjecting cell lysates to electrophoresis in agarose gels, followed by blotting to nitrocellulose membranes.
TNF cytotoxicity assay and Western blotting
After blotting, the PVDF membrane was stained with 2% amido black in 7% glacial acetic acid and the protein fronts of all lanes were compared for loading accuracy.
The urothelium contains a rich store of pre-formed MIF [ 10 11 ] . Moreover, MIF protein and mRNA were also detected by Western blotting techniques and RT-PCR in the major pelvic ganglia (MPG) that provides efferent (parasympathetic and sympathetic) innervation to the bladder and other pelvic viscera [ 12 13 ] . In addition, the L6-S1 dorsal root ganglia (DRG) that provide afferent innervation to the bladder [ 13 ] and other pelvic viscera constitutively expressed MIF.
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