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Example sentences for: blosum

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3. Maximum likelihood pairwise distance matrices are calculated for each of the x multiple alignments using a model of amino acid substitution (for example, BLOSUM [ 25 ] or Dayhoff PAM [ 26 ] ).
The recommended value of 5.0 times the residue diversity was used for the total number of pseudo-counts, and the amino acid substitution probabilities were taken from the BLOSUM 62 matrix [ 22 ] . A substitution matrix based on transmembrane helices [ 23 ] was also used in some cases, however, the results were not altered substantially.
The resultant six partial alignments were then combined by using with the profile alignment procedure of ClustalX [ 57 ] with the Blosum100 scoring matrix.
Maximum likelihood pairwise distances are calculated using BLOSUM matrices [ 25 ] by a modified version of TREE-PUZZLE [ 34 ] . Neighbor joining trees are calculated by a modified version of NEIGHBOR from the PHYLIP package [ 35 ] http://evolution.genetics.washington.edu/phylip.html.
In the work we describe here, alignments were done using a gap penalty of 10 and the BLOSUM62 scoring matrix [ 31].