Words similar to blots
Example sentences for: blots
How can you use “blots” in a sentence? Here are some example sentences to help you improve your vocabulary:
Triplicate slot blots were produced for all 24 genes present in Table 2. The concordance rate between the microarray and slot blot fold changes for the 24 genes was 83% overall but concordance depended on gene expression level (Table 2).
After denaturation in 6 M and renaturation in 0.187 M guanidine-HCl in HB buffer (25 mM Hepes pH 7.2, 5 mM NaCl, 5 mM MgCl2, 1 mM DTT), the blots were saturated at 4°C in buffer H (20 mM Hepes pH 7.7, 7.75 mM KCl, 0.1 mM EDTA, 25 mM MgCl 2 , 1 mM DTT, 0.05% NP40, 1% dry milk) for 2 hours, and then incubated for 2 hours at 4°C with 50 μl of 35S-methionine-labeled in vitro-translated hCCR4 or luciferase protein (synthesized using a reticulocyte lysate-coupled transcription/translation kit [Promega]).
The blots were probed with a 5' specific CaCET1 fragment (probe A in Figure 1), which detects both the wild-type CaCET1 allele and the cacet1::UAU1 allele, and with a 3'-specific CaCET1 fragment (probe B in Figure 1) derived from the segment deleted during construction of the cacet1::UAU1 disruption cassette.
Western blots
The blots were washed and exposed to an x-ray film (Kodak BioMax MS-2 film, Sigma) with an intensifying screen at -80°C for 4 days.