Example sentences for: blot

How can you use “blot” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The blocking peptide sc-6820 P (Santa Cruz Biotechnology) for N-19 ERβ antibody was mixed with the ERβ antibody at a 10:1 weight ratio, and incubated overnight at 4°C then 2 h at room temperature before the peptide-absorbed antibody was utilized for immunohistochemistry (PA/ERβ, Figure 2H) and western blot controls (PA/ERβ, Figure 6B).

  • Preliminary observations indicate that these BMP-5 transcripts are translated into protein in SCG in vivo as assessed by Western blot analyses using BMP-5 Ab (P.

  • To determine which key pathways were activated, Western blot analyses of phosphorylated or cleaved proteins in E15+2 CONT and SN50-treated explants were conducted as previously described [ 5 ] . For this set of experiments, we first determined the specificity for each of the following antibodies purchased from Cell Signaling Technology (Beverly, MA) using E15 and E17 SMG homogenates: anti-phosphorylated Erk1/2 [phospho-p44/42 MAP kinase (Thr202/Tyr204)] antibody, anti-phosphorylated c-Raf(Ser259) antibody, anti-cleaved Caspase 3 (D 175) antibody, and anti-cleaved PARP (D214) antibody.

  • Additionally, the results obtained from the RT-PCR and western blot analysis confirm our findings.

  • We conclude that the oligo hybridization patterns reflect transcript levels relatively accurately for the five genes presented in Figure 7. In fact, the representation is likely to be more accurate than that based on PCR products based on the RNA blot hybridization comparisons (Figure 6).


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