Example sentences for: blotted

How can you use “blotted” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After SDS-PAGED, proteins were blotted onto nitrocellulose membrane (PVDF Immobilon P Ø 0.45 μm, Millipore).

  • Samples of known numbers of cells from the cell lines and recombinant, purified Tag prepared from a baculovirus lysate [ 40 ] were loaded on 10% polyacrylamide discontinuous mini-gels (Bio-Rad, Hercules, CA) and electrophoresed conventionally [ 41 ] . Gels were then electrophoretically blotted [ 42 ] onto PVDF membrane (Bio-Rad) without methanol for 15 min at 100 V. Tag was visualized by immunostaining with PAb416 and alkaline phosphatase conjugated secondary antibody (Sigma) using chemiluminescent detection with CDP-Star substrate according to the manufacturers directions (Tropix, Bedford, MA).

  • At the conclusion of each experimental series, two samples (one control and one treated) were equilibrated in gadolinium-free medium, then extracted from media and blotted dry, and placed in an NMR tube.

  • Fractionated proteins were blotted onto nitrocellulose using the Transblot system as per manufacturer instructions (Biorad, Hercules, CA).

  • Memento Mori was about death; Reality and Dreams is about a sort of half death: being made redundant, being half blotted out.


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