Words similar to biogenex
Example sentences for: biogenex
How can you use “biogenex” in a sentence? Here are some example sentences to help you improve your vocabulary:
7% formaldehyde at -20°C for 20 minutes, then washed twice with 1 × PBS; the coverslips where then incubated with a 1:200 dilution of rabbit anti-cyclin D 1 antibody (Santa Cruz Biotechnology) or control rabbit sera (Biogenex) for 2 hours at room temperature followed by three washes with 1 × PBS; the coverslips were then incubated with a 1:250 dilution of rhodamine-labeled donkey-anti-rabbit anti-sera (Chemicon) for 45 minutes to detect the primary antibody; following three final washes with 1 × PBS, the coverslips were mounted on glass slides using ProLong Antifade (Molecular Probes) for fluorescent detection of GFP and cyclin D 1 on an Olympus IX70 microscope at 100 × magnification.
Hybridization mix is also available from other commercial companies such as BioGenex.
Four micron full coronal kidney sections from each animal were adhered to lysine-coated slides and used for immunodetection of phospho-Rb (Ser780) as follows: sections were deparaffinized and hydrated by placing in xylene for 8 minutes, followed by 3 minutes each of 100%, 96%, 80%, 70%, 50%, 30% ethanol, ending with 10 minutes in water; sections then underwent antigen retrieval (Glyca Reagent, Biogenex) followed by immunodetection (Super Sensitive Detection Kit, Biogenex) of phospho-Rb (Ser780) after a 12 hour incubation with a 1:1000 dilution of rabbit anti-phospho-Rb (Ser780) antibody (Cell Signaling) or control rabbit anti-sera (Biogenex) at 4°C.
The slides were washed with Optimax wash buffer (BioGenex, San Ramon, California, USA) after each incubation.
Sections from human pancreata were double stained for Ogg1 and somatostatin, as a marker for the islets, with rabbit anti-human Ogg1 (Novus Biologicals, Littleton, CO) and goat anti-human somatostatin antibodies (Santa Cruz Biotechnology, Santa Cruz, CA), after antigen retrieval by boiling for 10 min in acidic antigen retrieval solution (Biogenex, San Ramon, CA).
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