Words similar to biolabs

Example sentences for: biolabs

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Genomic DNA was treated with sodium bisulphite as previously described [ 33 ] . Placental DNA treated in vitro with Sss I methyltransferase (New England Biolabs, Beverly, MA) and similarly treated normal lymphocyte DNA were used as controls for methylated and unmethylated templates, respectively.
In blocking experiments, cells were preincubated with either a TGFβ 1 -RII-blocking antibody (R&D Systems # AF-241-NA, 15 μg/ml for 30 min), α V and β 6 -blocking antibodies (Santa Cruz, sc-6617 and sc-6632 respectively, 1:100 each for 30 min), or the MEK1 inhibitor PD98059 (New England Biolabs # 9900S, 7.5 ng/ml for 10 min) before stimulation with mature TGFβ 1 .
Cell clones were transiently transfected using Superfect reagent with 1 μg per well of 6 well plate with either pFR-luc (Promega) or pGAL4-SEAP (secreted alkaline phosphatase) (provided by Dr. R. Herzig, Rheogene Inc.) and 3 μg carrier DNA (pLITMUS, New England Biolabs).
ChIP DNA was fragmented by sonication and isolated using antibody against di-methyl-H3 K4 as described previously [ 6 ] . Restricted genomic DNA was prepared as follows: yeast genomic DNA isolated by bead lysis, phenol/chloroform extraction, and ethanol precipitation, was restricted either with Alu I or with Rsa I (New England BioLabs (NEB)).
Treatment of agarose slices linearized the plasmid DNA and allowed for plasmid size determination using a concatomerized bacteriophage lambda standard (New England BioLabs, Beverly, MA) (5).