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Example sentences for: biolabs

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The aligned small subunit ribosomal RNA sequences were retrieved from the Ribosomal Database Project II http://rdp.cme.msu.edu [ 44 ] . Francine Perler (New England Biolabs) the curator of the intein database http://www.neb.com/inteins/int_reg.htmlkindly provided the sequences of all known inteins.
Restriction enzymes were purchased from New England Biolabs (Boston, MA).
The PCR product was digested with SpeI (New England Biolabs, Beverly, MA; 20 units) and EcoRI (50 units) in buffer (50 mM NaCl, 100 mM Tris-HCl, pH 7.5, 10 mM MgCl 2 , 0.025% Triton X-100, 100 μg/ml BSA) at 37°C for 60 minutes, purified, and cloned into SpeI/EcoRI digested pTZtd1304.
The resulting product (459 bp) was subjected to Msp AI digest (New England Biolabs, Beverly, MA, USA) according to the manufacturer's directions.
In blocking experiments, cells were preincubated with either a TGFβ 1 -RII-blocking antibody (R&D Systems # AF-241-NA, 15 μg/ml for 30 min), α V and β 6 -blocking antibodies (Santa Cruz, sc-6617 and sc-6632 respectively, 1:100 each for 30 min), or the MEK1 inhibitor PD98059 (New England Biolabs # 9900S, 7.5 ng/ml for 10 min) before stimulation with mature TGFβ 1 .