Words similar to biolabs

Example sentences for: biolabs

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Anti-ERK1/2 (p44/p42) polyclonal antibody, anti-phosphorylated ERK1/2 polyclonal antibody, and horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG were purchased from New England Biolabs (Beverly, MA, USA).
PCR primer (200 ng) for probe sequence was end-labeled with 32Pγ-ATP and T4 polynucleotide kinase (New England Biolabs, Beverly, MA, USA), and was column purified.
500 ng (FAM labelling) or 1 μg (JOE and NED labelling) of the cDNA plasmid preparation was mixed with 0.5 pmol labelled oligonucleotides, 0.25 (FAM) or 0.5 (JOE and NED) Units Bgl I, and 10 (FAM) or 20 (JOE and NED) Units T4-DNA-ligase (New England Biolabs), respectively, in 20 mM Tris-acetate (pH 7.9), 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT, 100 μg/ml BSA, 1 mM ATP.
First-strand cDNA synthesis was primed with 4 μg of random nonamers (New England Biolabs) or 6 μg random hexamers (Life Technologies) by heating at 70°C for 10 minutes, snap-cooling in dry ice/ethanol for 30 sec, and incubating at room temperature for an additional 5-10 min.
200-400 ng of whole-cell DNA was denatured and annealed with an "Extend" primer using Vent (exo-) polymerase (New England BioLabs).