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Example sentences for: biolabs

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500 ng (FAM labelling) or 1 μg (JOE and NED labelling) of the cDNA plasmid preparation was mixed with 0.5 pmol labelled oligonucleotides, 0.25 (FAM) or 0.5 (JOE and NED) Units Bgl I, and 10 (FAM) or 20 (JOE and NED) Units T4-DNA-ligase (New England Biolabs), respectively, in 20 mM Tris-acetate (pH 7.9), 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM DTT, 100 μg/ml BSA, 1 mM ATP.
All restriction digests were performed according to manufacturer recommendations using enzymes purchased from New England Biolabs (Beverly, MA).
The proteins were transferred to nitrocellulose paper, and incubated at room temperature for 3 hours, or overnight, at 4°C, with mouse monoclonal anti-human Mcl-1 (1:1000), p38 MAP kinase, phosphoinositide 3 kinase (PI3K), or protein kinase B-alpha (PKB-α) (1:500) antibodies (Transduction Laboratories, San Diego, CA, USA and New England Biolabs, Inc., Beverly, MA, USA), or rabbit polyclonal anti-rat p44/42 MAP kinase (1:1000), or doubly phosphorylated anti-human Thr180/Tyr182 phospho-p38 MAP kinase (1:1000) antibodies (New England Biolabs, Inc.).
Electroporation of DNA into Y. pestis was done as described previously [ 32 ] . Gene amplification was performed with DeepVent (New England Biolabs, Beverly, MA) or Taq (Eppendorf Scientific, Westbury, NY) DNA polymerase in a Perkin-Elmer GeneAmp Model 2400 thermocycler (Applied Biosystems, Foster City, CA).
PhosphoCREB peptide (sequence KRREILSRRPS(P)YR) was obtained from New England Biolabs, MA.