Words similar to biolabs
Example sentences for: biolabs
How can you use “biolabs” in a sentence? Here are some example sentences to help you improve your vocabulary:
Genomic DNA was treated with sodium bisulphite as previously described [ 33 ] . Placental DNA treated in vitro with Sss I methyltransferase (New England Biolabs, Beverly, MA) and similarly treated normal lymphocyte DNA were used as controls for methylated and unmethylated templates, respectively.
Restriction enzymes were purchased from New England Biolabs and were used according to the supplied instructions.
Cell clones were transiently transfected using Superfect reagent with 1 μg per well of 6 well plate with either pFR-luc (Promega) or pGAL4-SEAP (secreted alkaline phosphatase) (provided by Dr. R. Herzig, Rheogene Inc.) and 3 μg carrier DNA (pLITMUS, New England Biolabs).
RNA (1 μg) was converted to cDNA in a 50 μl reaction containing 1 U of avian myeloblastosis virus reverse transcriptase (New England Biolabs, Beverly, MA, USA), 1 mM dNTP's, and 10 μg/ml random hexamer primers in 1× reverse transcriptase buffer at 42°C for 50 min; the reaction was terminated by heating to 90°C for 10 min.
ChIP DNA was fragmented by sonication and isolated using antibody against di-methyl-H3 K4 as described previously [ 6 ] . Restricted genomic DNA was prepared as follows: yeast genomic DNA isolated by bead lysis, phenol/chloroform extraction, and ethanol precipitation, was restricted either with Alu I or with Rsa I (New England BioLabs (NEB)).
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