Words similar to biolabs
Example sentences for: biolabs
How can you use “biolabs” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cell clones were transiently transfected using Superfect reagent with 1 μg per well of 6 well plate with either pFR-luc (Promega) or pGAL4-SEAP (secreted alkaline phosphatase) (provided by Dr. R. Herzig, Rheogene Inc.) and 3 μg carrier DNA (pLITMUS, New England Biolabs).
For each sample, 50 μL solution containing approximately 5 units of Klenow large fragment (New England Biolabs, Beverly, MA), 3 μg of single stranded binding protein (US Biochemicals, Cleveland, OH), and 0.5 μM Cy3- or Cy5-labeled dATP, dCTP, dGTP or dUTP (Perkin Elmer) was pipeted onto the gel.
The following antibodies were used in a dilution of 1:1,000: TGFβ 1 (Santa Cruz [sc], sc-146), p-Tyr (sc-7020), β 6 -integrin (sc-6632), α V -integrin (sc-6617), p125 FAK(sc-557), TGFβ 1 -RI (sc-402), TGFβ 1 -RII (sc-400-G), ERK1/2-P (sc-7383), SMAD2/3 (sc-6033), SOS1/2 (sc-259), p130 cas(UBI-06-500), PCNA (sc-56), p21 WAF1(sc-6246), p27 KIP(sc-1641), c-fos (sc-7202), c-jun (sc-44), raf1 (sc-133), p21 Ras(sc-35) and phospho-threonine antibody (New England Biolabs, # 9381).
Genomic DNA was treated with sodium bisulphite as previously described [ 33 ] . Placental DNA treated in vitro with Sss I methyltransferase (New England Biolabs, Beverly, MA) and similarly treated normal lymphocyte DNA were used as controls for methylated and unmethylated templates, respectively.
The PCR product was digested with SpeI (New England Biolabs, Beverly, MA; 20 units) and EcoRI (50 units) in buffer (50 mM NaCl, 100 mM Tris-HCl, pH 7.5, 10 mM MgCl 2 , 0.025% Triton X-100, 100 μg/ml BSA) at 37°C for 60 minutes, purified, and cloned into SpeI/EcoRI digested pTZtd1304.
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