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Example sentences for: biodesign

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Individual AV DNA titers were determined by three different methods: 1) plaque titration on 293 cells, 2) immunofluorescence microscopy of AV protein expression (anti-penton group antigen, clone 143, Biodesign, Kennebunk, ME) in 293 cells infected with serial dilutions of AV and 3) absorbance at 260 nm (pfu/ml = A
The following antibodies and sera were used, normal rabbit serum (Vector Laboratories Inc., Burlingame, CA), rabbit polyclonal anti-SOD1 [ 86 ] , a second rabbit polyclonal anti-SOD1 (Biodesign, Saco, ME) and a mouse monoclonal antibody against CO1.
Trypsin: An anti-human cationic trypsin antibody was used at 1:1000 (Biodesign, Kennebunk, ME).
Sections were processed for antibody staining using the following antibodies: rabbit [ 86 ] and sheep (BioDesign) anti-SOD1 (both recognize mouse and human SOD1), mouse anti-cytochrome c oxidase subunit CO1 and CO4 (Molecular Probes, OR), rabbit anti-TOM20 and TOM40 (Santa Cruz Biotechnology, CA), mouse anti-cytochrome c (BD PharMingen, San Diego, CA), sheep anti-catalase (The Binding Site, Birmingham, England), rabbit anti-catalase (Calbiochem, SanDiego, CA), rabbit anti-PMP70 (ABR, Golden, CO or Axxora, SanDiego, CA), and rabbit anti-cathepsin D (DAKO, Carpinteria, CA) according to the protocol described previously [ 26 28 ] . The stained sections were examined and digitized using a confocal microscope (Leica TCS-SP).