Example sentences for: antimouse

How can you use “antimouse” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Blots were washed and incubated with secondary antibody (1:1500 horseradish peroxidase) conjugated antimouse (eNOS) or antirabbit (nNOS, iNOS, and β-actin) for 45 min at 37°C.

  • Sections of fetal and residual maternal ovarian tissues were incubated for 30 min with 10% normal goat serum and for 1 h with primary antibodies (1 μg/ml), washed in two changes of PBS, incubated for 30 min with secondary goat antirabbit (F0382) or antimouse (F0257) immunoglobulin G-fluorescein isothiocyanate (FITC) (1:40), and washed in two changes of PBS.

  • After washing, bound antibody isotope was detected with biotin-conjugated antimouse whole IgG (heavy and light chain) antibody (Pharmingen, San Diego, CA, USA).

  • Slides were washed and incubated with goat antimouse biotinylated antibody at a 1 : 750 dilution followed by incubation with streptavidine horseradish peroxidase.

  • 1 μg protein/100 μl per well) for ELISA, and free binding capacity of the wells was blocked by 1% fat-free milk in phosphate-buffered saline [ 23 24 25 ] . Sera were applied at increasing dilutions and isotypes of proteoglycan-specific antibodies were determined using peroxidase-conjugated rat antimouse IgG 1 or IgG 2a secondary antibodies (Zymed, South San Francisco, CA, USA) as previously described [ 24 26 ] . Serum antibody levels were calculated according to mouse IgG 1 and IgG 2a standards.


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