Words similar to antimutator
Example sentences for: antimutator
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Recombinant viruses were successfully obtained for all pols except wild type HSV-1 SC16 and the low error rate or antimutator HSV-1 PAA r5 [ 10 ] . The pol -recombinant viruses were plaque-purified and the pol genes completely sequenced along with those of their parental viruses to confirm the recombination event.
HSV-1 strain SC16 (wild type laboratory virus), HSV-2 SB5 (wild type laboratory virus), HSV-2 6652 (wild type clinical virus), HSV-2 83D (wild type clinical virus), HSV-2 6757 (high error mutant clinical virus) were described previously [ 21 ] and the HSV-1 PAA r5 (antimutator laboratory virus) utilized in this study was a kind gift of D. Coen (Harvard University, Boston, MA) [ 10 ] . HP66 (HSV-1 genome containing a LacZ insertion within the pol coding region) viral DNA was kindly provided by D. Coen (Harvard University, Boston, MA) [ 17 ] . HP66 viral stocks were prepared by transfecting HP66 viral DNA into PolB3 cells, which allow for virus propagation by expressing the viral polymerase.
The presence of an antimutator phenotype with this viral polymerase in the tk mutagenesis assay was not apparent when using the non-HSV DNA mutagenesis assay, and therefore phenotype-labelling may be dependent upon both the assay method and/or the target gene.
The control viruses, HSV-2 6757 and HSV-1 PAA r5, demonstrated mutator or antimutator phenotypes, respectively, in the tk mutagenesis assay, but not in the non-HSV DNA mutagenesis assay.
Thus, classification of a pol gene as mutator (HSV-2 6757) or antimutator (PAA r5) is not a general characteristic, but is likely dependent upon the assay method and target gene.
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