Words similar to antimitotic
Example sentences for: antimitotic
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This work fulfills their suggestion to quantify changes in tubulin isotype levels with the appropriate antibodies, and establishes a sensitive and accurate assay to complement mRNA assays and studies of the response of cultured cells to antimitotic drugs.
In most experiments, endogenous non-neuronal cells were eliminated from cultures by adding cytosine-β-D-arabinofuranoside (Sigma, St. Louis, MO) to the culture medium at 1 μM for 48 hr beginning on day 2. In some experiments this antimitotic was not added to cultures but rather endogenous non-neuronal cells were allowed to proliferate.
The expression of genes encoding α-tubulin and β-tubulin seems to be developmentally regulated [ 19 ] . For example, tubulin isotype expression patterns change during the development and maturation of neurons [ 14 20 ] . Studies with paclitaxel or other antimitotic agents, such as colchicine or estramustine, indicate that drug interactions with tubulin isotypes differ [ 21 22 23 24 25 ] and might contribute to cell resistance to antimitotics [ 26 27 28 29 30 31 ] . However, one group of antimitotic agents, the vinca alkaloids, was shown to bind with similar affinity to all tubulin isotypes [ 12 ] , suggesting that alterations in drug binding affinity is unlikely to be the cause of drug resistance.
Furthermore, transfection of CHO cells with β-tubulin classes I, II, or IVb did not produce resistance to paclitaxel [ 32 ] . The alternative hypothesis, derived from the work of Cabral and colleagues, is that rather than the tubulin isotype levels, the amount of polymerized tubulin is the critical factor that regulates response to antimitotic drugs.
It was demonstrated that one group of antimitotic agents, vinca alkaloids, bind with similar affinity to all β-tubulin isotypes [ 12 ] , thus suggesting for therapy with vinorelbine, vinblas-tine, or vincristine that tubulin isotype levels are incidental for efficacy.
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