Words similar to aggregating
Example sentences for: aggregating
How can you use “aggregating” in a sentence? Here are some example sentences to help you improve your vocabulary:
Nagy et al demonstrated that embryos and mice could be derived solely from R1 ES cells by aggregating them with tetraploid embryos [ 7 8 ] . Although we were able to repeat these data using R1 ES cells we were unable to generate midgestation embryos from E14TG2a-derived strains of hprt negative ES cell lines using the same approach [ 9 10 11 ] . We believed it was unlikely that Hprt itself was required to generate ES cell-derived embryos because hprt knockout mice are viable and fertile [ 12 ] . E14TG2a-derived ES cell lines contain a large spontaneous deletion encompassing 55 kb of the hprt locus [ 13 ] . Therefore, it seemed possible that sequences within this region were necessary to maintain ES cell pluripotency.
However, if a line of "transgenic" ES cells can be established then it is possible to generate clonal embryos directly from these ES cells by aggregating them with tetraploid embryos.
We next addressed whether embryos could be generated solely from F3 Hprt negative ES cells by aggregating these cells with tetraploid CD-1 embryos and allowing them to develop in utero until day 13 of gestation (El 3).
This causes the break-up of the aggregating stream into groups of the normal number of cells [ 43 ] . Thus, the large size of the structures formed by developing BS153 cells can be accounted for by the lower extracellular Countin concentration and thus a lack of breaking up into smaller groups of cells.
However, pioneering work by Nagy et al has shown that embryos and mice can be derived directly from R1 ES cells by aggregating them with tetraploid embryos [ 7 8 ] . This suggests that controlled expression of transgenes encoding possible gain-of-function and developmental-lethal alleles could be achieved by generating embryos and/or mice from ES cells containing transgenes inserted into the hprt locus.
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