Words similar to zas-c

Example sentences for: zas-c

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The fusion proteins KRC/ZAS-N and KRC/ZAS-C were produced in E. coli and purified by affinity chromatography as described previously [ 24 28 ] . The regions of KRC used to generate KRC/ZAS-N and KRC/ZAS-C are schematically shown in Fig.
The oligonucleotide pool (~500 ng) was incubated with KRC/ZAS-N or KRC/ZAS-C (100 μg each) and 10 μg of non-specific competitor DNA poly(dI-dC).
Passes of MEME for pentameric motifs yielded homologous TG-rich sequences for the KRC/ZAS-N and KRC/ZAS-C datasets: T(T/G)T(T/G)G and GGTAT for KRC/ZAS-N, and TGTGG/T for KRC/ZAS-C.
Similarly, the ZAS-C pentamer together with its complement form the canonical RSS heptamer palindrome "CACTGTG".
With respect to the heptamer (canonical sequence: CACAGTG), the CAC sequence bordering the recombination site is the most conserved segment of the sequence [ 32 ] , and mutation of these nucleotides has been found to decrease V(D)J joining in transfection assays using recombination substrates [ 40 ] . Because the sequence of the canonical RSS heptamer is palindrome, we speculate that the TGTG sequence may be sufficient for KRC/ZAS-C binding.