Words similar to zas-c

Example sentences for: zas-c

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KRC/ZAS-N or KRC/ZAS-C (100 μg each; Fig.
The differences in the linker regions, TGERP for ZAS-N and TDVRP for ZAS-C, which presumably interact with the sugar-phosphate backbone of DNA, and the observation that KRC/ZAS-C more readily forms higher-ordered structures with DNA than KRC/ZAS-N may also contribute to the differences in the DNA binding of ZAS-N and ZAS-C.
Passes of MEME for pentameric motifs yielded homologous TG-rich sequences for the KRC/ZAS-N and KRC/ZAS-C datasets: T(T/G)T(T/G)G and GGTAT for KRC/ZAS-N, and TGTGG/T for KRC/ZAS-C.
Since the RSS heptamer is a palindrome, it is possible for two KRC molecules to bind to an RSS heptamer, with one ZAS-C binding to the top strand and the other ZAS-C binding to the bottom strand.
Subsequently, sequence analysis identified KRC as a member of the ZAS family of proteins which share the ability to bind κB-like motifs [ 15 ] . DNA competition analysis showed that KRC fusion proteins containing the ZAS-C domain bind specifically to both the RSS and to the κB motif [ 14 28 ] . DNA footprinting analysis further showed that KRC/ZAS-C binds to specific nucleotides within the κB and the heptamer of the RSS [ 14 ] . In this study, using a PCR-based DNA-binding site-selection and amplification procedure, we demonstrated that both the N-terminal ZAS-N and the C-terminal ZAS-C domains are able to bind GT-rich DNA sequences, and confirmed that the RSS and κB motifs are the high-affinity targets of KRC.