Words similar to zas-n
Example sentences for: zas-n
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The fifty-three KRC/ZAS-N-selected sequences (ZAS-N dataset) and 49 KRC/ZAS-C-selected sequences (ZAS-C dataset) were first analyzed by the Motif Expectation Maximum for Motif Elicitation (MEME) computer program.
Subsequently, sequence analysis identified KRC as a member of the ZAS family of proteins which share the ability to bind κB-like motifs [ 15 ] . DNA competition analysis showed that KRC fusion proteins containing the ZAS-C domain bind specifically to both the RSS and to the κB motif [ 14 28 ] . DNA footprinting analysis further showed that KRC/ZAS-C binds to specific nucleotides within the κB and the heptamer of the RSS [ 14 ] . In this study, using a PCR-based DNA-binding site-selection and amplification procedure, we demonstrated that both the N-terminal ZAS-N and the C-terminal ZAS-C domains are able to bind GT-rich DNA sequences, and confirmed that the RSS and κB motifs are the high-affinity targets of KRC.
We had previously shown by methylation interference analysis that KRC/ZAS-C bound specifically to the sequence TGTGG within the context of the canonical RSS heptamer plus the immediately flanking guanine [ 14 ] . Because the pentamer motif for KRC/ZAS-C predicted by MEME completely matched with the empirical results, we conclude that the two pentameric motifs discovered by MEME are likely authentic binding sites for KRC/ZAS-N as well.
The oligonucleotide pool (~500 ng) was incubated with KRC/ZAS-N or KRC/ZAS-C (100 μg each) and 10 μg of non-specific competitor DNA poly(dI-dC).
The fusion proteins KRC/ZAS-N and KRC/ZAS-C were produced in E. coli and purified by affinity chromatography as described previously [ 24 28 ] . The regions of KRC used to generate KRC/ZAS-N and KRC/ZAS-C are schematically shown in Fig.
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