Words similar to zas-n
Example sentences for: zas-n
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It is possible that two KRC molecules may be needed to interact with an RSS nonamer: one protein whose ZAS-N domains may bind to the 5'-half of an RSS nonamer while a second protein's ZAS-N domain may bind to the 3'-half of an RSS.
The fifty-three KRC/ZAS-N-selected sequences (ZAS-N dataset) and 49 KRC/ZAS-C-selected sequences (ZAS-C dataset) were first analyzed by the Motif Expectation Maximum for Motif Elicitation (MEME) computer program.
Furthermore, in rounds four and five, a cluster of close migrating DNA-protein complexes were observed for KRC/ZAS-N (Fig.
In the site-selection experiment, the increasing yield of DNA-protein complexes in successive rounds of DNA amplification and purification suggest that KRC/ZAS-N and KRC/ZAS-C bound DNA specifically.
After cloning and DNA sequencing, the KRC-selected sequence datasets were analyzed by the computer program Multiple Expectation Maximum for Motif Elicitation (MEME version 3.0) to generate sets of position specific scoring matrices (PSSMs) or motifs [ 30 ] . When the program was set to identify wider sequences (= 9 nucleotides) the PSSMs were homologous to Sb [ 29 ] , the κB motif [ 31 ] , and the canonical heptamer and nonamer elements of the RSS [ 32 ] . However, shortening the width of the motifs to 5 nucleotides, the target length for the C 2 H 2 zinc finger pairs of the tramtrack [ 33 ] , the ZAS-N dataset yielded two motifs, "GGTAT" and "T(T/C)TT(T/G)G" and the ZAS-C dataset yielded a single motif, "TGTGG".