Example sentences for: ypop
How can you use “ypop” in a sentence? Here are some example sentences to help you improve your vocabulary:
Second, yCCR4 was shown to be a component, in association with yPOP2, of the major yeast cytoplasmic mRNA deadenylase complex, and to be required for efficient poly(A)-specific mRNA degradation [ 14 15 ] . yCCR4 might be a catalytic subunit of this complex, because of its nuclease-like domain.
Indeed, both human homologs of yPOP2, hCAF1 and hPOP2, lack the 148 N-terminal amino acids which are, in yPOP2, required for transcriptional activation, whereas the yPOP2 C-terminus, which is involved in the interaction with yCCR4, is conserved in hCAF1 [ 11 ] and hPOP2 [ 19 ] . Similarly, hNOT2, the human homolog of the yNOT2 component of the CCR4-NOT complex, harbors a N-terminal domain divergent from that of yNOT2 -required for transcriptional activation and interaction with the yeast ADA2 factor-, but has conserved the C-terminal domain which is absolutely required for the yCCR4-associated function of yNOT2 [ 19 20 ] . Therefore, it looks as if genes of the CCR4-NOT complex have undergone a "concerted" phylogenetic evolution, with the yeast genes encoding multifunctional proteins and the animal genes only encoding "specialized" proteins specific for the CCR4-NOT complex.
Interestingly, we found that hCCR4 also binds to the second human yPOP2 paralog, hCAF1.
To determine whether yCCR4-yPOP2 interactions are evolutionarily conserved, we examined whether hCCR4 can bind hCAF1 and hPOP2 in a two-hybrid assay in mammalian cells.
Here we report the identification of nineteen yCCR4-like proteins in eukaryotes and show, by phylogenetic and genomic analyses, that they are grouped into four distinct families, one of which contains the yCCR4 orthologs : these orthologs, in animals, have conserved the yCCR4 leucine-rich repeat, and we show, using two-hybrid assays and far-Western experiments, that the human protein binds to the human yPOP2 homologs, i.e. hCAF1 and hPOP2, in a LRR-dependent manner.
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