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Example sentences for: yvt

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Plasmids were retested by isolation from yeast using the phenol/glass bead method followed by electroporation into bacteria [ 32 ] . The plasmids were then isolated from bacteria using a commercial DNA miniprep kit (Qiagen) and re-transformed in to yVT87.
Transcriptionally active peptides were selected by transforming yVT87 with the appropriate peptide library and selecting for transformants on plates lacking histidine.
Yeast strain yVT87 (EGY48 (Clontech) MATa, ura3, his3, trp1, leu2::lexA6op- LEU2 ) was transformed by the method of Gietz and Schiestl (Agatep, R., R.D.