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Example sentences for: yvt

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Yeast strain yVT87 (EGY48 (Clontech) MATa, ura3, his3, trp1, leu2::lexA6op- LEU2 ) was transformed by the method of Gietz and Schiestl (Agatep, R., R.D.
Plasmids were retested by isolation from yeast using the phenol/glass bead method followed by electroporation into bacteria [ 32 ] . The plasmids were then isolated from bacteria using a commercial DNA miniprep kit (Qiagen) and re-transformed in to yVT87.
Transcriptionally active peptides were selected by transforming yVT87 with the appropriate peptide library and selecting for transformants on plates lacking histidine.