Words similar to untransfected
Example sentences for: untransfected
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IL-6 given exogenously, since LNCaP cells do not produce IL-6, resulted in increased growth of the cells concomitant with activation of STAT3 [ 36 ] . LNCaP cells transfected with a plasmid conferring constitutive IL-6 expression demonstrated increased growth, relative to untransfected or sham-transfected cells [ 36 ] . However, other investigators have observed that IL-6 treatment of LNCaP cells reulted in terminal differentiation and inhibition of growth, associated with STAT3 activation [ 17 37 38 ] . The molecular basis for the apparent contradiction is unknown at this time.
In the first control experiment, untransfected CHO cells were utilized to determine if any of the SP conjugates were eliciting Ca ++responses through a pathway independent of rSPR activation.
When 1 nM or 10 nM Alexa 488-SP was added the fluorophore produced signals similar in intensity to those observed when this analog was added to transfected or untransfected CHO cells (data not shown).
Similarly, membrane staining was observed in transfected CHO cells, but not in untransfected cells.
There was no indication of cell death in the pon A treated cultures and pon A had no effect on the untransfected parental MCF7 cell line (Fig.
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