Words similar to tyrosine-phosphorylated
Example sentences for: tyrosine-phosphorylated
How can you use “tyrosine-phosphorylated” in a sentence? Here are some example sentences to help you improve your vocabulary:
Gefitinib inhibited the activity of wild-type and L858R EGFRs progressively with increasing concentrations of drug, as demonstrated by a reduction of tyrosine-phosphorylated proteins (Figure 4A) and a decrease in p-EGFR:t-EGFR ratios (Figure 4B).
The filters were washed with T-TBS for 30 min and incubated with horseradish-peroxidase conjugated secondary antibodies, and tyrosine-phosphorylated proteins were visualized by enhanced chemiluminescence.
Cells were stimulated with increasing concentrations of IGF-I, and whole cell lysates immunoblotted for tyrosine-phosphorylated proteins.
In this report, we questioned whether the EGFR acts as a surrogate scaffold during ERK activation through the δ-OR, as it appears to do for several other GPCRs [ 7 ] . To act as a surrogate-binding site for complex formation, the literature states that this protein must first become tyrosine-phosphorylated [ 7 ] . Tyrosine phosphorylation of the EGFR is apparent after agonist stimulation of the α 2 -AR (COS-7), β 2 -AR (COS-7), insulin-like growth factor (IGF-1; COS-7), substance P, and the lysophosphatidic acid receptor (LPAR) [ 7 ] . In addition, each of the aforementioned ligands also increases ERK phosphorylation when the proper receptor is expressed in these cell lines.
Phospho-tyrosine phosphatase assays were performed similarly to previously described protocols [ 14 58 ] . For the preparation of tyrosine-phosphorylated substrate, 7 × 10 6HTC-IR cells [ 59 ] were incubated with medium containing insulin (50 nM/ml) and lysed with RIPA buffer.
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