Words similar to tyrosines
Example sentences for: tyrosines
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The adaptor protein Cas has a N-terminal SH3 domain, a large number of tyrosines that serve as potential kinase substrates within its substrate domain and a C-terminal Src binding domain (Fig.
PR was obtained in 85% purity, assuming that values of ε 280 and ε 546 for pR are the same as for bR solubilized in DMPC/cholate/SDS mixtures at pH 8 (ε 280 = 7.85 × 10 4cm -1M -1and ε 551 = 4.8 × 10 4cm -1M -1) [ 5 ] . This assumption is actually expected to underestimate the purity of pR produced, by up to ~20%, since the pR we expressed has 10 tryptophan and 14 tyrosine residues, as compared to 8 tryptophans and 11 tyrosines in bR from H. salinarum.
Cas is a protein which migrates with an apparent mass of approximately 130 kDa, with a N-terminal SH3 domain, a central substrate domain (SD) with up to 15 tyrosines which can be phosphorylated, a C-terminal region with a proline-rich region, and two tyrosine phosphorylation sites capable of binding Src kinases (Src binding domain (SBD)) [ 6 18 ] . The latter SBD domain regulates the ability of Src to activate the serum response element, as well as in the phosphorylation of the SD of Cas by Src [ 18 19 20 ] . The substrate domain controls adhesion and migration and the majority of the tyrosines within this domain are of the sequence YQXP (5) or YDXP (10), which when phosphorylated, bind to the adaptors c-crk, nck and AND-34 [ 16 21 22 23 ] . The importance of these molecules in Cas function is unclear, however c-crk seems to be critical in the regulation of adhesion and migration mediated by Cas.
The corresponding control peptide with tyrosines instead of sulfotyrosines, and N36 (SGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARVL) and C34 (WIEWDREINNYTSIIYSLIEESQNQQEKNEQELL) peptide constituents of the gp41 core [ 20 21 ] of HIV-1 BaL were from AnaSpec, Inc., San Jose, CA.
Immunoblotting for the presence of phosphorylated MAPK (ERK1/2) and phosphorylated tyrosines
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