Words similar to tta
Example sentences for: tta
How can you use “tta” in a sentence? Here are some example sentences to help you improve your vocabulary:
The Q758C variant was frequently found adjacent to the wild-type T7 RNA polymerase promoter (GACT at positions -11 through -8) but was also found adjacent to a single mutant promoter (GAC G ), double mutant promoters (GA TA , G T C A ), a triple mutant promoter (G TTA ), and a promoter in which all the residues differed from wild-type ( TGTA ). Overall, the 'generalist' polymerase appeared to prefer mutant promoters that contained a T at position -9, and G or A at -8 positions.
1: GAT C TC GAT CCC GCG AAA TTA ATA CCA CTC ACT ATA GGG GAA TTG TGA GCG GAT AAC AAT TCC CCT (BglII site in bold)
For NRG1, the oligos used were HZ034, 5' TCG ACC AGC ATA TTA CTA CCC TTC GCA AAC TTT CAG GCA CTG TGC GGT ATT TCA CAC CG 3'; and HZ035, 5' GTA GTA CTG CTA ATG AGA AAA ACA CGG GTA TAC CGT CAA AGA TTG TAC TGA GAG TGC AC 3'.
For MIG2, the oligos were HZ045, 5' TGA CCT CGA GAA CAA ACA AAA TAA AAA TAA AAA AAG AGA CTG TGC GGT ATT TCA CAC CG 3'; and HZ046, 5' TTA GAG GAA AAA TGG TGA GAT AAA AAG GGG CCG TAA AGG AGA TTG TAC TGA GAG TGC AC 3'.
Each of the megaprimers was purified and subsequently amplified with GL1 primer (5' TGT ATC TTA TGG TAC TGT AAC TG 3'), using StuI B or HincII B plasmid as a template.