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Example sentences for: tsd

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This step was necessary because L1s can be, and often are, part of larger segmental duplications in the genome; in this case, identity between the downstream sequences of two such L1s cannot be attributed to 3' transduction without significant analysis by hand, and the sequence identity will generally continue well beyond the 3' TSD.
These TSDs were selected by the TSDfinder program in part because the 3' TSD was preceded by a poly(A) tail (see Materials and methods).
To exclude the possibility that the abundance of the 11-20-nucleotide long TSDs in our collection is exclusively a result of this aspect of the scoring scheme, TSDfinder analysis of this dataset was repeated without rewarding a higher value to TSDs of length > 10 nucleotides, and the distribution of TSD lengths remained generally unchanged.
To investigate this, we recreated pre-insertion loci by collecting 50 nucleotides of upstream and downstream flanking sequence and the TSD sequence of each L1 insertion for which a high-scoring TSD was found.
For five of the transduced sequences lacking a clear polyadenylation signal, the alignment between the 3'-transduced sequence and family members ended at an A-rich sequence; the 3' TSD of the 3'-transduction event was found immediately downstream of this A-rich sequence in the DNA (Figure 3b).