Words similar to ts

Example sentences for: ts

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Subsequently, the wells were washed and postcoated for 1 h at 20°C with bovine serum albumin (BSA) and gelatin (1 and 0.1 mg/ml in 0.14 M NaCI, 0.01 M Tris, pH 7.0 [TS]).
TAF II function in living cells has been studied in yeast where the use of temperature sensitive (TS) mutants has shown that many TAF II s are required for transcription of the majority of yeast genes [ [ 13 14 15 16 17 ] ]. In contrast, TS lesions in TAF II 145, TAF II 150, and TAF II 90 have a less dramatic effect affecting the expression of only a specific subset of genes mainly involved in the cell cycle [ [ 18 19 ] ] (for reviews see [ 3, 20].
Subfragment d as shown if Fig 7Dlane 10 can bind strongly to the 35 kDa protein, however mutation altering the A and Ts of the core ACS sequence to G and Cs (Fig 7C) to generate the dmut oligonucleotide results in the abolition of its ability to bind p35.
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A fraction of the total cellular Sec13p is isolated in a NPC subcomplex containing Nup84p, Nup85p, Nup120p, Nup145p-C, and Seh1p (a Sec13-related protein) [ 40 41 ] . Hurt and coworkers have also reported that ts sec13 mutants show enhanced growth defects when combined with a nup85Δ mutant, and result in the mislocalization of GFP-Nup49p to NE clusters and cytoplasmic foci [ 41 ] . This correlates with our observations of GFP-Nic96p and Nup170p-GFP in our sec13/npa2 mutant.