Words similar to triphosphates
Example sentences for: triphosphates
How can you use “triphosphates” in a sentence? Here are some example sentences to help you improve your vocabulary:
For each hybridization, 2 μg of each purified sample of polyadenylated RNA was reverse-transcribed in the presence of fluorescently labeled nucleoside triphosphates (Cy3-dUTP for reference sample, Cy5-dUTP for experimental sample).
50 μl amplification reactions contained 100-200 ng DNA template and 45 μl of a reaction mix (200 μM each, deoxynucleotide triphosphates; 1.0 μM each primer; 1.25 U Taq polymerase; 10 mM Tris hydrochloride (pH 8.3); 50 mM KCl; 1.5 mM MgCl 2 ; 0.01% gelatin).
Deoxynucleotide triphosphates (dNTP's), MgSO 4 , thermopol buffer, Deep Vent DNA polymerase, and restrictions enzymes were purchased from Promega or New England Biolabs.
To our knowledge, no systematic studies have been performed examining the incorporation of modified ribonucleotide triphosphates into cRNA, yield of amplified material, effects on hybridization intensities and reproducibility, and the impact on the differential expression ratios.
Polymerization of ribonucleoside triphosphates (NTPs), which is central to a variety of crucial biological processes, including transcription, primer synthesis during DNA replication, addition of polyA tails to mRNA, addition of CCA to tRNA, uridylation in RNA editing, replication of RNA viruses, amplification of RNA in eukaryotic post-transcriptional gene silencing (PTGS), and oligoadenylate synthesis during interferon signaling, is catalyzed by a broad variety of distinct enzymes [ 1 2 3 4 ] . These polymerases belong to two major mechanistic categories, those that copy a nucleic acid template and those that are independent of a template.
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