Words similar to triphosphatases
Example sentences for: triphosphatases
How can you use “triphosphatases” in a sentence? Here are some example sentences to help you improve your vocabulary:
Here we have shown that the RNA triphosphatases Pct1 and CaCet1 are essential for viability of S. pombe and C. albicans, respectively The conclusion that CaCet1 is essential is based on a finding that none of the 54 independent isolates in the single-transformation test were homozygous for cacet1Δ; our interpretation is consistent with criteria established by Mitchell and colleagues for inference of essentiality using this genetic approach.
Analogous active sites, that coordinate two metal ions, are observed in domains with similar activities, such as the classical adenylyl/guanylyl cyclases, family B DNA polymerases, pol-β fold nucleotidyl transferases, and triphosphatases or phosphoesterases of the HD and DHH superfamilies [ 11 15 19 20 ] . Consistent with these observations, both CyaB and ThTPase have been shown to require Mg 2+ions for their nucleotide cyclase and phosphatase activities [ 16 17 ] .
RNA triphosphatase is an attractive therapeutic target for fungal infections because: (i) the active site structure and catalytic mechanism of fungal RNA triphosphatase are completely different from the RNA triphosphatase domain of the metazoan capping enzyme and (ii) metazoans encode no identifiable homologs of the fungal RNA triphosphatases.
Using sensitive sequence profile comparison methods, we show that the CyaB-like adenylyl cyclases are homologs of the soluble mammalian thiamine triphosphatases [ 17 ] , and they define a novel superfamily of enzymes that utilize ATP and other organic phosphates.
To address these issues, we have characterized the RNA triphosphatases of two other fungi, including the human pathogen Candida albicans and the fission yeast Schizosaccharomyces pombe [ 10 11 12 ] . The fungal triphosphatases, S. cerevisiae Cet1, C. albicans CaCet1 and S. pombe Pct1, belong to a new family of metal-dependent phosphohydrolases that embraces the triphosphatase components of DNA virus and protozoan mRNA capping systems [ 1 7 13 14 ] . The defining features of the metal-dependent RNA triphosphatases are two glutamate-containing motifs that are required for catalysis and comprise the metal-binding site in the crystal structure of S. cerevisiae Cet1.
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