Words similar to triphosphatase
Example sentences for: triphosphatase
How can you use “triphosphatase” in a sentence? Here are some example sentences to help you improve your vocabulary:
This frequency of homozygosity at a nonessential locus is similar to that reported by Mitchell's group (2 out of 30) for homozygous disruption of the C. albicans CDC25 gene [ 16 ] . These results confirm that the single-transformation test can, in our hands, be used to identify a nonessential gene and they underscore the inference from the data presented here that RNA triphosphatase is essential for growth of C. albicans.
Here we constructed pct1Δ and pce1Δ plasmids containing 5' and 3' flanking genomic sequences in which the entire triphosphatase or guanylyltransferase coding sequence was deleted and replaced by the kanamycin resistance gene [ 17 ] . The pct1::kanMX and pce1::kanMX constructs were transformed separately into a diploid strain of S. pombe and chromosomal integrants containing one copy of the wild-type gene and one of pct1::kanMX or pce1::kanMX were selected on medium containing G418.
This is not a straw-man issue, given that S. cerevisiae encodes two homologous RNA triphosphatases (Cet1 and Cth1), of which only Cet1 is essential for capping and cell viability [ 8 15 ] . We use classical genetic approaches to show that the respective genes encoding RNA triphosphatase and RNA guanylyltransferase are essential in S. pombe.
We named this domain the CYTH ( Cy aB, th iamine triphosphatase) domain after the two experimentally characterized proteins in which it is present.
However, the atomic structures and catalytic mechanisms of the fungal and mammalian RNA triphosphatases are completely different [ 2 3 ] . Thus, it has been suggested that RNA triphosphatase is a promising target for antifungal drug discovery [ 2 ] .
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