Words similar to triphosphatase
Example sentences for: triphosphatase
How can you use “triphosphatase” in a sentence? Here are some example sentences to help you improve your vocabulary:
Based on these findings, and the presence of a Cet1 homolog in the Apergillus fumigatus proteome, we conclude that RNA triphosphatase is a valid target for antifungal drug development.
The triphosphatase (Cet1), guanylyltransferase (Ceg1), and methyltransferase (Abd1) components of the capping apparatus are essential for cell growth in the budding yeast S. cerevisiae [ 1 4 5 6 ] . Mutations of the RNA triphosphatase Cet1 that abrogate catalytic activity in vitro are lethal in vivo [ 7 8 9 ] ; thus, it is reasonable to think that pharmacological inhibition of Cet1 function in vivo would impede cell growth.
[ 16 ] to test gene function in diploid C. albicans, we were unable to disrupt both copies of the CaCET1 gene, signifying that RNA triphosphatase is also essential in that species, a significant human pathogen.
Mitchell and colleagues [ 16 ] have developed a single-transformation method to test gene function in diploid C. albicans that entails the following steps, which we have applied to the CaCET1 gene encoding RNA triphosphatase [ 22 ] .
Here we constructed pct1Δ and pce1Δ plasmids containing 5' and 3' flanking genomic sequences in which the entire triphosphatase or guanylyltransferase coding sequence was deleted and replaced by the kanamycin resistance gene [ 17 ] . The pct1::kanMX and pce1::kanMX constructs were transformed separately into a diploid strain of S. pombe and chromosomal integrants containing one copy of the wild-type gene and one of pct1::kanMX or pce1::kanMX were selected on medium containing G418.
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