Words similar to triphosphatase
Example sentences for: triphosphatase
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Although the three capping reactions are universal in eukaryotes, there is a surprising diversity in the genetic organization of the capping enzymes as well as a complete divergence in the structure and catalytic mechanism of the RNA triphosphatase component in "lower" versus "higher" eukaryotic species [ 1 ] . Metazoans and plants have a two-component capping system consisting of a bifunctional triphosphatase-guanylyltransferase polypeptide and a separate methyltransferase polypeptide, whereas fungi contain a three-component system consisting of separate triphosphatase, guanylyltransferase, and methyltransferase gene products.
This frequency of homozygosity at a nonessential locus is similar to that reported by Mitchell's group (2 out of 30) for homozygous disruption of the C. albicans CDC25 gene [ 16 ] . These results confirm that the single-transformation test can, in our hands, be used to identify a nonessential gene and they underscore the inference from the data presented here that RNA triphosphatase is essential for growth of C. albicans.
This scenario is plausible only if RNA triphosphatase is essential for growth of pathogenic fungi that cause human disease (e.g.
Here we address the critical question of whether RNA triphosphatase is essential for cell growth in fungal species other than S. cerevisiae.
To address these issues, we have characterized the RNA triphosphatases of two other fungi, including the human pathogen Candida albicans and the fission yeast Schizosaccharomyces pombe [ 10 11 12 ] . The fungal triphosphatases, S. cerevisiae Cet1, C. albicans CaCet1 and S. pombe Pct1, belong to a new family of metal-dependent phosphohydrolases that embraces the triphosphatase components of DNA virus and protozoan mRNA capping systems [ 1 7 13 14 ] . The defining features of the metal-dependent RNA triphosphatases are two glutamate-containing motifs that are required for catalysis and comprise the metal-binding site in the crystal structure of S. cerevisiae Cet1.
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