Words similar to triphosphate

Example sentences for: triphosphate

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For each PCR run, a master mixture was prepared on ice with 1× Platinum PCR buffer (Gibco/Life Technologies, Grand Island, NY, USA), 1.5 mM MgCl 2 , 0.2 mM of each deoxynucleoside triphosphate, 0.2 μM each primer, 2.5 units platinum Taq DNA polymerase (Gibco/Life Technologies, Grand Island, NY, USA).
Ecto-Nucleoside triphosphate diphosphohydrolases (E-NTPDases, formerly called ecto-ATPases) hydrolyze nucleotides in the presence of divalent cations and are insensitive to inhibitors of P-type, F-type, and V-type ATPases [ 1 ] . Three isoforms that differ in the ratio of ATPase/ADPase activity are present on the cell surface [ 2 ] : E-NTPDase1 with a ratio of 1, E-NTPDase2 with a ratio of 10 and E-NTPDase3 with a ratio of 3-5.
The metal cations direct a 5' nucleoside triphosphate to form a phosphoester bond with the 3' hydroxyl of the preceding nucleotide (the 3'-terminal nucleotide of a growing polynucleotide chain), with the elimination of pyrophosphate [ 6 ] .
Apoptosis was assessed by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) (In situ Cell Death Detection Kit, POD, Boehringer-Manheim).
Cyclic AMP generation and thiamine triphosphate hydrolysis appear to be secondarily acquired activities.