Example sentences for: triphosphate

How can you use “triphosphate” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The metal cations direct a 5' nucleoside triphosphate to form a phosphoester bond with the 3' hydroxyl of the preceding nucleotide (the 3'-terminal nucleotide of a growing polynucleotide chain), with the elimination of pyrophosphate [ 6 ] .

  • 43% sodium triphosphate, 47.

  • When diadenosine 5',5"'-( P 1, P 2-methylene- P 3-thio)- P 1, P 3-triphosphate [ 17 ] was crystallized with wild-type and mutant Fhit, the α-phosphorothioate group was found in the mutant but not wild-type active site, suggesting that α-phosphorothioate inhibitors may be slow substrates [ 12 ] . Similarly, Frey and co-workers found that Fhit slowly cleaves both R p and S p stereoisomers of γ-( m -nitrobenzyl)-adenosine 5'- O -(l-thiotriphosphate) with modest and similar beneficial effects on K m as compared to the corresponding phosphate [ 13 ] .

  • Our results suggest that incorporation of 2-aminoadenosine (DAP) triphosphate into target cRNA samples may increase hybridization signal intensity for a wide variety of RNA:DNA hybrid duplexes.

  • Recently, studies employing DNA arrays have also examined the use of modified nucleotides such as DAP, 5-bromodeoxyuridine, and 2'-O-methylthymidine in the probe [ 21 22 ] . An alternative method to increase the probe-target affinity is to incorporate the modified ribonucleotide, as the triphosphate derivative, during the IVT process so that the cRNA produced has the desired level of substitution of the corresponding unmodified nucleotide.


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