Words similar to transgene-positive
Example sentences for: transgene-positive
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Approximately the same yield of gene-positive clones had been previously observed during TAR cloning of this genomic region with vectors lacking ARS and a counter-selectable marker [ 7 15 ] . No transgene-positive clones were found when 5-FOA selection was omitted (Table 3), indicating that the new TAR cloning method is highly selective and efficient for isolation of multicopy genes.
, one that uses a vector lacking ARS and a counterselectable marker) yields about 1%-2% transgene-positive transformants [ 7 15 ] , this result demonstrates that the specific targeting hook recombines much less efficiently when it is protected by a ~ 1.1 kb nonhomologous URA3 sequence.
No transgene-positive clones were found among the 1,000 transformants analyzed.
Eight transgene-positive YAC clones were identified among 950 His +5-FOA-resistant transformants screened by PCR for the presence of the zeta-globin promoter sequence (Table 3).