Words similar to transcriptase
Example sentences for: transcriptase
How can you use “transcriptase” in a sentence? Here are some example sentences to help you improve your vocabulary:
5G-YMHA contains mutations in the reverse transcriptase motif YMDD of P (D513H and D514A) that ablate DNA polymerization [ 17].
Reverse transcription was carried out in a 20 μl volume containing 1 μg total RNA, 1 μg oligdT25- T7 (5'-AAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGG -CGATT-3') primer, 1 μg template switch primer (5'-AAGCAGTGGTAACAACGC -AGGGACCGGG-3'), 4 μl first-strand reaction buffer, 2 μl 10 mM dithiothreitol (DTT; Gibco-BRL) 1 μl 10 mM dNTPs, 1 μl SUPERase-in, (Amtion, Austin, TX) and 200 u Superscript II reverse transcriptase (Gibco- BRL).
FFPE = formalin-fixed, paraffin-embedded; H&E = hematoxylin and eosin; IHC = immunohistochemistry; LCM = laser capture microdissection; OPN = osteopontin; PBS = phosphate-buffered saline; PCR = polymerase chain reaction; qPCR = real-time, quantitative PCR; RT = reverse transcriptase; TSP-1 = thrombospondin-1; TYRP-1 = tyrosinase-related protein-1.
The recovered mRNA was reverse-transcribed using AMV-reverse transcriptase (Amersham Pharmacia Biotech Inc., Newark, NJ) and the primer gc3'pET.
One microgram of RNA was reverse transcribed to complementary (c)DNA using 200U Moloney murine leukemia virua (M-MLV) reverse transcriptase (GIBCO BRL Life Technologies, Grand Island, NY, USA), 1U RNAse inhibitor (5 Prime 3 Prime, Boulder, CO, USA) and 20 pmol oligo dT primer in a total volume of 20 μl.