Words similar to transcriptase
Example sentences for: transcriptase
How can you use “transcriptase” in a sentence? Here are some example sentences to help you improve your vocabulary:
The programmed cell death gene, PDCD6 , is a member of the family of intracellular Ca2 +-binding proteins and a part of the apoptotic machinery controlled by T-cell receptor (TCR), Fas, and glucocorticoid signals [ 11 17 ] . TERT encodes a reverse transcriptase required for the replication of chromosome termini and plays a role in telomere elongation.
Reverse transcriptase activity in viral cores was assayed by incubating cores in endogenous polymerase buffer with 40 μM dATP, dCTP, dTTP and 2 μCi [α 32P]dGTP (Amersham) at 37°C for 90 min.
AMPA, α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid; DMEM, Dulbecco's modified Eagle medium; FBS, fetal bovine serum certified; FCC, ferricytochrome c type III; HBSS, Hank's balanced salt solution; LPS, lipopolysaccharide; MMP-9, matrix metalloproteinase-9; O 2 -, superoxide; P, penicillin; PBS, phosphate buffered saline; PMA, phorbol 12-myristate 13-acetate ; RT-PCR, reverse transcriptase polymerase chain reaction; S, streptomycin; SOD, superoxide dismutase; TNF-α, tumor necrosis factor.
For RT-PCR, 1 μg of total RNA was reverse-transcribed into cDNA using SuperscriptII reverse transcriptase (Invitrogen, Carlsbad, CA).
The generalized group II intron secondary structure consists of six helical domains emerging from a central wheel [ 1 2 ] . Some group II introns are capable of self-splicing; however for efficient splicing in vivo , the intron-encoded ORF or host-encoded splicing factors are required [ 3 ] . Mobile group II introns encode a reverse transcriptase/maturase, with the coding region generally located in domain IV.