Example sentences for: transcriptase

How can you use “transcriptase” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Six microliters of 5x first-strand buffer (RGI) (1 μl 100 mM DTT, 1.5 μl dNTPs (20 mM), 1.5 μl reverse transcriptase (Superscript II, Gibco BRL), 10 μl [ 33P]dCTP (ICN)) were then added to the primed RNA.

  • Among extant biological entities, these polymerases are encoded largely by RNA viruses and retroid viruses, but a reverse transcriptase had been recruited by eukaryotes as the catalytic subunit of the telomerase [ 98 99 ] . There seems to be a distinct possibility that both classes of RDRPs are vestiges of the ancient RNA world that have been largely displaced from cellular biochemical machinery at an early stage of evolution, because of the deleterious effects of RNA replication in DNA-based organisms, while surviving in selfish genetic elements.

  • Understanding the mechanism of the template/substrate commitment of the hepadnaviral reverse transcriptase may need to wait until a three-dimensional structure of the polymerase bound to a nucleic acid primer-template is obtained.

  • Semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) analysis

  • Extensive controls lead us to believe that the RNAseH active site is most probably "substrate committed" in a manner similar to the "template commitment" of the reverse transcriptase activity.


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