Words similar to transcriptase

Example sentences for: transcriptase

How can you use “transcriptase” in a sentence? Here are some example sentences to help you improve your vocabulary:

RNA (1 μg) was converted to cDNA in a 50 μl reaction containing 1 U of avian myeloblastosis virus reverse transcriptase (New England Biolabs, Beverly, MA, USA), 1 mM dNTP's, and 10 μg/ml random hexamer primers in 1× reverse transcriptase buffer at 42°C for 50 min; the reaction was terminated by heating to 90°C for 10 min.
To distinguish between these alternatives, hnRNP A2/B1/Neo fusion transcripts expressed in MEF and ES cells were analyzed by reverse transcriptase PCR (RT-PCR).
1 μg of total RNA was then analysed by RT-PCR performed as described [ 50] Control reactions (not shown) contained all components except reverse transcriptase.
Moreover, a recent study found that both cDNA and oligodeoxyribonucleotide arrays underestimated the relative changes in mRNA expression between experimental and control samples, as determined by quantitative reverse transcriptase polymerase chain reaction [ 29 ] . This underestimation (or ratio compression) increased as the relative change increased, consistent with our observations (Figure 4B).
Two micrograms of the extracted RNA was treated with 2 U DNaseI to eliminate DNA, and reverse transcribed with 200 U SuperScriptII reverse transcriptase at 42°C for 70 min using oligo dT primers in accordance with the manufacturer's instructions.