Example sentences for: transcribed

How can you use “transcribed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • RNA was reverse transcribed into cDNA using random hexamer primers in the presence of 5-aminoallyl-dUTP; Cy3 and Cy5 dyes were covalently coupled to the incorporated aminoallyl linkers in a subsequent labeling reaction.

  • Filters 1, 2 and 3 were hybridized with cDNA reverse transcribed from 2 and 6 μg of pig, and 4 μg of human total RNA, and filters 4, 5 and 6 were hybridized with cDNA reverse transcribed from 4 μg of pig total RNA (see Table 1).

  • Total RNA samples (1 μg) were reverse transcribed to yield first-strand cDNA using the Applied Biosystems Reverse Transcription Reagents protocol (Applied Biosystems, Foster City, CA, USA).

  • To assess gene expression, total RNA isolated from normal testes, the cell lines, and tumor tissues, and polyA+ RNA of testis obtained from Clontech (Palo Alto, CA) was reverse transcribed using random primers and the Pro-STAR first strand RT-PCR kit (Stratagene, La Jolla, CA).

  • The purified PCR product (5 μl) was transcribed in 96-well format by incubation for 2 h at 37°C after the addition of 5 μl of a polymerase reaction cocktail consisting of 2 U T7 RNA polymerase, 4.6 U RNase inhibitor, 10 mM NTPs, 3.5 mM digoxigenin-11-UTP, 40 mM Tris pH 8.0, 6 mM MgCl 2 , 10 mM DTT and 2 mM spermidine.


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