Words similar to transcribed
Example sentences for: transcribed
How can you use “transcribed” in a sentence? Here are some example sentences to help you improve your vocabulary:
RNA was reverse transcribed into cDNA using random hexamer primers in the presence of 5-aminoallyl-dUTP; Cy3 and Cy5 dyes were covalently coupled to the incorporated aminoallyl linkers in a subsequent labeling reaction.
Filters 1, 2 and 3 were hybridized with cDNA reverse transcribed from 2 and 6 μg of pig, and 4 μg of human total RNA, and filters 4, 5 and 6 were hybridized with cDNA reverse transcribed from 4 μg of pig total RNA (see Table 1).
Total RNA samples (1 μg) were reverse transcribed to yield first-strand cDNA using the Applied Biosystems Reverse Transcription Reagents protocol (Applied Biosystems, Foster City, CA, USA).
To assess gene expression, total RNA isolated from normal testes, the cell lines, and tumor tissues, and polyA+ RNA of testis obtained from Clontech (Palo Alto, CA) was reverse transcribed using random primers and the Pro-STAR first strand RT-PCR kit (Stratagene, La Jolla, CA).
The purified PCR product (5 μl) was transcribed in 96-well format by incubation for 2 h at 37°C after the addition of 5 μl of a polymerase reaction cocktail consisting of 2 U T7 RNA polymerase, 4.6 U RNase inhibitor, 10 mM NTPs, 3.5 mM digoxigenin-11-UTP, 40 mM Tris pH 8.0, 6 mM MgCl 2 , 10 mM DTT and 2 mM spermidine.