Words similar to transcribed
Example sentences for: transcribed
How can you use “transcribed” in a sentence? Here are some example sentences to help you improve your vocabulary:
Small samples (2 μg) from each RNA fraction were reverse transcribed and radiolabeled (2 μg oligo dT, 1x First Strand buffer, 5 mM DTT, 75 U SuperscriptII [Life Technologies], 20 mM dNTP mix containing dATP, dGTP, dTTP and 100 mCi 33P-dCTP (ICN Radiochemicals).
One was to see if the ATM mRNA is properly transcribed, and another is to directly see the ATM protein itself by Western blotting analysis from the tumors and compare with the normal form.
A different 3' oligonucleotide primer, designed from a divergent portion of the 3'-untranslated region of each of the different MHC genes, was used in each reaction tube to provide isoform specificity (sequences in [ 19 22 ] ). Template, in addition to 5 μg of reverse transcribed cDNA, included 1 pg of an internal control fragment [ 22 ] to be coamplified with each PCR by using the same primers and target sequences.
To demonstrate that all predicted murine TMC genes are transcribed, we performed RT-PCR experiments.
For each sample, 2 μg RNA was transcribed in a 20 μl reaction using the Invitrogen SuperScript First-Strand Synthesis System oligo-(dT) primer method according to the manufacturer's instructions.
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