Words similar to tk
Example sentences for: tk
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Two-hybrid protein-protein interaction assays were performed in Hela cells, with a reporter plasmid (pG4-TK-Luc) containing six GAL4 binding elements upstream of the thymidine kinase (TK) promoter region fused to the luciferase reporter gene (Fig.
The natural phenomenon of spontaneous mutation, which occurs in the absence of drug selection, results in the accumulation of approximately six to eight TK-deficient variants per 10 4plaque-forming viruses in virus populations that have never been exposed to selective pressure [ 6 10 12 ] . Furthermore, HSV-2 clinical isolates were shown to have a higher frequency of spontaneous mutations resistant to ACV and PCV, approximately 30-fold, compared to HSV-1 isolates, and the majority of these mutations are in tk . Consistent with this observation, HSV-2 strains also exhibited approximately a 20- to 80-fold higher spontaneous mutation rate to cidofovir (HPMPC), an inhibitor of HSV Pol, resistance compared with HSV-1 [ 21 ] . Therefore, these naturally-occuring mutations are not unique to tk and TK substrates as detected with antiviral agents ACV and PCV, but were extended with the direct Pol inhibitor, HPMPC, to other loci, most likely pol .
The pol from HSV-2 6757, a virus which showed a 300- to 700-fold higher error rate than HSV-1 SC16 by the tk mutagenesis assay previously [ 21 ] , was found to contain three mutations compared to the other HSV-2 pols , with one change residing in region VII which is shared among diverse DNA polymerases (Fig.
Since the control viruses described above functioned as expected in the tk mutagenesis assay, recombinant viruses expressing the HSV-2 polymerase within an HSV-1 genome were also examined.
In particular, the co-sedimentation analyses suggest that the TK mutant may be severely impaired in the ability to bind acidic phospholipids generally.
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