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Example sentences for: tk-

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The percent of added TK-1 cells that adhered to the SVEC monolayers was quantified as follows: %adhesion = [cpm in lysate /(cpm in lysate + cpm in supernatant and wash)] × 100.
The TK-1 lymphocyte cell line used in this assay expresses high levels of the α4β7 integrin, [ 39 41 ] which can interact with multiple ligands including mucosal addressin-1 (MAdCAM-1), as well as VCAM-1, L-selectin and fibronectin [ 42 ] . In this system, TNF-α stimulated TK-1 adhesion to SVEC4-10 endothelial cells is at least 50% MAdCAM-1 dependent [ 41 ] . SVEC monolayers were grown in 48-well plates as described, and to activate endothelium, the monolayers were incubated with TNF-α (1 ng/ml) for 24 h.
Labeled TK-1 cells were then added to the endothelium at a 5:1 lymphocyte to endothelial cell ratio [ 43 ] and allowed to bind for 30 min under static conditions.
TK-1 lymphocyte adhesion assay
Having established a role of NO donors in the regulation of MAdCAM-1 expression by endothelial cells, we examined effects of NO donors on the adhesion of a4b7 expressing mouse lymphocyte cell line TK-1 to TNF-α-treated endothelial cultures.