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Example sentences for: tk-

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The TK-1 lymphocyte cell line used in this assay expresses high levels of the α4β7 integrin, [ 39 41 ] which can interact with multiple ligands including mucosal addressin-1 (MAdCAM-1), as well as VCAM-1, L-selectin and fibronectin [ 42 ] . In this system, TNF-α stimulated TK-1 adhesion to SVEC4-10 endothelial cells is at least 50% MAdCAM-1 dependent [ 41 ] . SVEC monolayers were grown in 48-well plates as described, and to activate endothelium, the monolayers were incubated with TNF-α (1 ng/ml) for 24 h.
Labeled TK-1 cells were then added to the endothelium at a 5:1 lymphocyte: endothelial cell ratio [ 6] and allowed to bind for 30 min under static conditions.
7 ± 5.4% as many of the TK-1 lymphocytes bound to SVEC compared to TNF-α stimulation (100%, or 'maximal' binding).
Briefly, TK-1 cells were suspended in culture medium and fluorescence labeled by incubating TK-1 cells at 2 × 10 6cells/ml with 0.02 mg fluorescein diacetate (FDA) (Sigma) at 37°C for 30 min.
TK-1 lymphocyte adhesion assay