Words similar to tk-luc
Example sentences for: tk-luc
How can you use “tk-luc” in a sentence? Here are some example sentences to help you improve your vocabulary:
The DNA for transfection included 100 ng of pG4-TK-Luc reporter plasmid together with 50 ng of GAL4 and/or VP16 fusion vectors, and 10 ng of pCMV-RL vector (cytomegalovirus promoter fused to the renilla luciferase reporter gene [Promega]) as an internal control for transfection efficiency.
The βRARE-TK-Luc reporter plasmid with three copies of the DR-5 motif from the promoter of the mouse RARβ2 gene has been described previously, as has the CYP2B10-TK-Luc reporter with two copies of the DR-4 type elements from the promoter of the mouse CYP2B10 gene and the CYP3A4-TK-luc reporter with three copies of the ER-6 element from the human CYP3A4 promoter [ 16 ] . Expression vectors for full length mCAR and RXR proteins were as described [ 9 ] . The cdm8-mCARΔ8 deletion construct containing a deletion of the last 8 amino acids and the mutant with 2 helix 3 mutations (F171L, I174A) that block ligand binding and response have been described previously [ 16 ] , as has the cdm8-RXRΔ19 construct with a deletion of the last 19 amino acids [ 3 ] .
Two-hybrid protein-protein interaction assays were performed in Hela cells, with a reporter plasmid (pG4-TK-Luc) containing six GAL4 binding elements upstream of the thymidine kinase (TK) promoter region fused to the luciferase reporter gene (Fig.
4B, co-expression of pGal4-hCCR4 with either pVP16-hCAF1 or pVP16-hPOP2 elicited a significant increase (> 35 fold) in pG4-TK-Luc expression, thus demonstrating association between hCCR4 and either hCAF1 or hPOP2.
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