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Example sentences for: tkt
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The astrocyte lineage of the LTR lines has been published [ 6 13 ] . The astrocyte lineage of the tkT lines was confirmed by the presence of the astrocyte-specific intermediate filament protein, GFAP [ 14 ] (Figure 1).
Cortical astrocytes were harvested from neonatal C57BL/6 mice and infected with a replication-defective retrovirus encoding Tag and neomycin resistance as previously described [ 13 ] . The transferred viruses were SV40-6 [ 5 ] , in which wild type Tag is expressed from the M-MuLV LTR promoter; SV(X)T K1, which is constructed the same as SV40-6 except that the Tag is mutant [ 38 ] and defective for binding the retinoblastoma protein family [ 39 ] ; Linker-tkT, in which the cDNA for Tag is expressed from the herpes simplex 1 tk promoter [ 11 ] . The differentiation and transformation phenotypes of the cell lines utilizing the M-MuLV LTR promoter have been described [ 6 13 ] . The Linker-tkT lines (tkT) were either isolated as clones after G418 selection (as previously described) [ 6 ] or frozen immediately after selection (pooled clones) and clonally selected at a later time.
The remaining two essential gene products were in the pentosephosphate pathway ( tktAB, rpiAB ). The tktAB and rpiAB gene products were required for the synthesis of erythrose 4-phosphate in all phases (aromatic amino acid supplement required for the cultivation of tkt - E. coli mutant strains [ 27]).
We have previously characterized clonal mouse astrocyte cell lines, immortalized by transduction of SV40 large T antigen expressed from the MoMuLV LTR [ 6 13 ] . Here, we compare variation in Tag levels in 3 of these lines and 3 additional lines (termed tkT lines), immortalized with Moloney Sarcoma Virus vectors expressing Tag from an internal herpes thymidine kinase (tk) promoter.
Characterization of tkT lines
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