Words similar to thymidine
Example sentences for: thymidine
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DNA-bound 3H-thymidine was immobilized on glass fiber filter paper (Packard, Meriden, CT) with the aid of an automated cell harvester (Tomtec, Orange, CT) and the tritium incorporation was determined by scintillation counting.
Cell lines were created by incorporation of retroviral vectors into the genome and expression of Tag from either the retroviral LTR (considered to be a strong promoter) or the herpes virus thymidine kinase promoter, internal to the viral LTR [ 1 ] . In our established NIH 3T3 cell line, Tag is expressed from the htk promoter at considerably lower levels than from the LTR for a considerable time after transduction [unpublished observations, and [ 11 ] ]. However, when a large number of htk-Tag astrocyte clones were checked 100 days after creation, the expression levels displayed a very large range, and thus we expect that this weak promoter is subject to positional effects on transcription or mutational events that affect gene expression (unpublished data).
The ability of the CHO-MK cells to incorporate thymidine in response to IGF-I is markedly diminished as compared to the parental cell line (Figure 1B).
Androgen is essential for normal prostate function, but has also been implicated in the pathogenesis and neoplasia of prostate cancer [ 1 2 26 ] . On the other hand, retinoid is essential for normal prostate function [ 7 27 28 ] and exhibits anti-proliferative and chemopreventive efficacy in experimental prostate carcinoma [ 8 9 10 ] . The dose-dependent stimulation of 3H-thymidine incorporation in LNCaP cells by T and RA when administered alone, and reciprocal suppression of such effects by each other, are consistent with previous findings [ 29 30 31 32 ] . These results illustrate interactions between androgen and retinoid in the control of LNCaP cell growth.
Moreover, all five clones were defective for TK activity compared with HSV-1 SC16 in a 3H-thymidine kinase assay (Table 4).
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