Words similar to thymidine
Example sentences for: thymidine
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For cytokines assays, 1 × 10 6transduced cells were incubated on anti-CD3 coated plates for 72 h at 37°C/5% CO 2 . Culture supernatants were then harvested and an ELISA assay was performed to measure the production of IL-4, IL-10 and IFN-γ as previously described [ 30 ] . To assay proliferation, the transduced cells were plated in duplicate in microtiter plates (5 × 10 5/well) at a ratio of 1:5 with irradiated BALB/c splenocytes and the indicated doses of OVA peptide at 37°C/5% CO 2 . After 72 h, 50 μCi/ml [ 3H]thymidine (Amersham, Piscataway, NJ) was added to the well.
Resistance to acyclovir (ACV) or penciclovir (PCV) generally arises by a single base mutation in either the HSV thymidine kinase (TK) or DNA polymerase (Pol) gene [for a review, see reference [ 5 ] ]. Unlike Pol, TK is not essential for virus replication in cell culture [ 7 ] , although in vivo analyses implicate it in HSV virulence, pathogenicity and reactivation from latency [ 4 8 16 22 ] . Since ACV and PCV are dependent upon HSV TK for monophosphorylation, an event obligatory for the formation of the active triphosphate inhibitor [ 14 ] , ACV risolates often exhibit high levels of cross-resistance to PCV [ 2 3 ] .
Cell proliferation was measured by [ 3H]-thymidine uptake [ 8 ] . HeLa S3 cells (3 × 10 4cells/well) were incubated in 96-well plates in the presence of varying concentrations of the tetrapyrroles for 2 days at 37°C, after which 3H-TdR (1 μCi/well) was added.
The ability of the CHO-MK cells to incorporate thymidine in response to IGF-I is markedly diminished as compared to the parental cell line (Figure 1B).
Freshly isolated PBMCs (10 5 cells) were incubated with baculovirus-derived recombinant p24 protein (Protein Sciences, Meriden, Connecticut, United States) at 5 μg/ml for 6 d and then pulsed with 3 H thymidine at 1.0 μCi for 6 h before harvesting as previously described [20].
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