Words similar to thermopol
Example sentences for: thermopol
How can you use “thermopol” in a sentence? Here are some example sentences to help you improve your vocabulary:
The ERCA mix contained 5% tetramethyl ammonium oxalate [ 21 ] , 400 μM dNTP mix, 1 uM each of the two primers, 8 u of Bst polymerase (New England Biolabs, MA), and 1X modified ThermoPol reaction buffer containing 20 mM Tris-HCl (pH 8.8), 10 mM KCl, 10 mM (NH 4 ) 2 SO 4 and 0.1% Triton X-100.
Deoxynucleotide triphosphates (dNTP's), MgSO 4 , thermopol buffer, Deep Vent DNA polymerase, and restrictions enzymes were purchased from Promega or New England Biolabs.
The reaction was cooled to 4°C, and 20 μl ERCA reaction mix was added (typically 16 units BST polymerase (New England Biolabs, Beverly, MA), 6 mM dNTPs, 0.5 μM P1, 0.5 μM P2, 4 μM Q-PNA, 7.5 μM TMAO in 1x ThermoPol Buffer II, all concentrations final).