Words similar to thermostable
Example sentences for: thermostable
How can you use “thermostable” in a sentence? Here are some example sentences to help you improve your vocabulary:
For example, a compartmentalized self-replication has been reported for the selection of thermostable variants of Taq DNA polymerase.
Single nucleotide discrimination is achieved at the ligation step by the use of the thermostable DNA ligase, Ampligase, which has a high affinity for a perfectly matched substrate at the 3' end of a DNA molecule [ 17 ] . We were able to enhance allele discrimination at the ligation step by designing the OCP such that the 5' complementary region is firmly hybridized to the target sequence whereas the 3' region is in equilibrium with its target at the ligation temperature [ 12 ] . This would result in increased specificity since the correctly matched OCP will have a greater chance to act as substrate for the ligase.
The first step in the genotyping assay involves SNP interrogation by ligation of the OCP using the thermostable ligase, Ampligase™, which is reported to have excellent discrimination between a matched and mismatched 3' terminal nucleotide [ 17 ] . In order to directly investigate the efficiency and specificity of the ligation step, allele discrimination of OCP ligation was analyzed using 5' 32P-labeled OCP.
In general terms, the optimization of this technical approach required particular attention to the design of the adaptamers, the choice and use of the thermostable DNA polymerase, and the thermocycling conditions.
First, two different thermostable polymerases ( Taq and Pwo polymerase) were used to generate the primary amplification products for cloning and subsequent sequence analysis.
Loading...