Example sentences for: thermocycler

How can you use “thermocycler” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The unaffected male amplicon and the mix were heated to 95°C for 5 min. and cooled slowly over 45 min. to 25°C in a thermocycler.

  • A positive sample was defined as previously described, as 5 times the uncorrected optical density (OD) of a sample containing no template DNA, but with 100 fg of internal control [ 8 ] . The controls were designed to allow for cutoff determination, as well as to determine the success of the PCR thermocycler run.

  • Reaction and thermocycler conditions were the same as before except gene specific primer 2, TMOD4RACE5'.

  • Thermocycler conditions were as follows for a Perkin Elmer 9600 thermocycler: 94°C for 4 min, followed by 35 cycles of 94°C for 30 s, annealing temperature (see Table 2) for 30 s, 72°C for 1 min, and a final extension of 72°C for 10 min.

  • Samples were amplified for 30 cycles in a GeneAmp PCR system 2400 thermocycler (Applied Biosystems) with a three-step cycle of denaturation for 30 s at 94°C, annealing for 30 s at 55°C to 70°C (optimized for each primer pair), and extension for 1 min at 72°C.


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