Example sentences for: thermocycler

How can you use “thermocycler” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Reactions were carried out in PCR plates and incubated in an ABI thermocycler according to the manufacturer's instructions.

  • PCRs were performed in a model 9600 Thermocycler (Perkin-Elmer Cetus) using the following cycling protocol: initial denaturation at 94°C for 10 min; 94.

  • Thermocycler conditions for a PE9600 were as follows: 94°C for 5 min, followed by 10 cycles of 94°C for 30 s, 71°C for 30 s, 72°C for 4 min; followed by one cycle of 94°C for 30 s, 68°C for 4 min 20 s, followed by 17 additional cycles each adding 20 s to each extension; final extension at 72°C for 10 min. 5 μl of a 1:50 dilution of the product from the first RACE reaction served as template for the second nested RACE reaction.

  • The PCR for the competitive ELISA analysis was conducted using NV-1 and NV-2 oligonucleotides specific for the Ssp I repeat as previously described by Bockarie and others [ 8 ] . Amplification was performed with an Applied Biosystems 9700 PCR thermocycler (Branchburg, NJ, USA) using 36 cycles with Amplitaq gold (Applied Biosystems Branchburg, NJ, USA) in a reaction volume of 50 μl.

  • Samples were then thermocycled for PCR amplification (Hybaid, Touchdown Thermocycler, Middlesex, United Kingdom) according to the following reaction conditions: 1 min 95°C, 1 min 55°C, 2 min 72°C (25-30 cycles).


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