Words similar to thermocycler
Example sentences for: thermocycler
How can you use “thermocycler” in a sentence? Here are some example sentences to help you improve your vocabulary:
The unaffected male amplicon and the mix were heated to 95°C for 5 min. and cooled slowly over 45 min. to 25°C in a thermocycler.
A positive sample was defined as previously described, as 5 times the uncorrected optical density (OD) of a sample containing no template DNA, but with 100 fg of internal control [ 8 ] . The controls were designed to allow for cutoff determination, as well as to determine the success of the PCR thermocycler run.
Reaction and thermocycler conditions were the same as before except gene specific primer 2, TMOD4RACE5'.
Thermocycler conditions were as follows for a Perkin Elmer 9600 thermocycler: 94°C for 4 min, followed by 35 cycles of 94°C for 30 s, annealing temperature (see Table 2) for 30 s, 72°C for 1 min, and a final extension of 72°C for 10 min.
Samples were amplified for 30 cycles in a GeneAmp PCR system 2400 thermocycler (Applied Biosystems) with a three-step cycle of denaturation for 30 s at 94°C, annealing for 30 s at 55°C to 70°C (optimized for each primer pair), and extension for 1 min at 72°C.