Example sentences for: thermocycler

How can you use “thermocycler” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • PCR was done on PE9600 or PE9700 thermocycler.

  • The unaffected male amplicon and the mix were heated to 95°C for 5 min. and cooled slowly over 45 min. to 25°C in a thermocycler.

  • The reactions were done in the thermocycler PTC 200 (MJ Research).

  • A positive sample was defined as previously described, as 5 times the uncorrected optical density (OD) of a sample containing no template DNA, but with 100 fg of internal control [ 8 ] . The controls were designed to allow for cutoff determination, as well as to determine the success of the PCR thermocycler run.

  • Thermocycler conditions for a PE9600 were as follows: 94°C for 5 min, followed by 10 cycles of 94°C for 30 s, 71°C for 30 s, 72°C for 4 min; followed by one cycle of 94°C for 30 s, 68°C for 4 min 20 s, followed by 17 additional cycles each adding 20 s to each extension; final extension at 72°C for 10 min. 5 μl of a 1:50 dilution of the product from the first RACE reaction served as template for the second nested RACE reaction.


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