Example sentences for: thermocycler

How can you use “thermocycler” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Samples were amplified for 30 cycles in a GeneAmp PCR system 2400 thermocycler (Applied Biosystems) with a three-step cycle of denaturation for 30 s at 94°C, annealing for 30 s at 55°C to 70°C (optimized for each primer pair), and extension for 1 min at 72°C.

  • PCR reactions were performed in a MJ Research thermocycler.

  • PCR amplifications of cDNA inserts were carried out at annealing temperatures of 50°C (614 and 606 libraries) or 60°C (707 library) in a 25 μl volume in a GeneAmp PCR system 9700 (Applied Biosystems, Foster City, CA) thermocycler for 35 cycles with a 2 min extension time.

  • A positive sample was defined as previously described, as 5 times the uncorrected optical density (OD) of a sample containing no template DNA, but with 100 fg of internal control [ 8 ] . The controls were designed to allow for cutoff determination, as well as to determine the success of the PCR thermocycler run.

  • Reactions were carried out in PCR plates and incubated in an ABI thermocycler according to the manufacturer's instructions.


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast