Words similar to tgemd

Example sentences for: tgemd

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Protein was step-eluted off of the resin with TGEMD buffer containing increasing concentrations of NaCl: 0.2 M, 0.5 M and 1.0 M. Most of the HA-NFATp was found in the 1.0 M NaCl fraction and was highly purified (shown in Figure 1C).
The recombinant protein was purified on anti-HA-conjugated beads and washed two times in TGEMD buffer (20 mM Tris (pH 7.9), 20% (v/v) glycerol, 1 mM EDTA, 5 mM MgCl 2 , 0.1% NP-40, 0.2 mM PMSF, 1 μg/ml leupeptin, 1.4 μg/ml pepstatin A, and 1 mM DTT) containing 1 M NaCl and an additional two times in TGEMD buffer containing 0.2 M NaCl.
This was followed by extensive washes in TGEMD (1.