Words similar to tetramer-positive
Example sentences for: tetramer-positive
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A large number of clonal CTL lines (more than 200) were generated by FACS of individual G209–2M- and M26-tetramer-positive cells directly from PBMC samples (Table 2).
RNA was extracted from clones and tetramer-positive cells using TRIzol (Invitrogen, Carlsbad, California, United States) and reverse-transcribed into cDNA using SuperScript II Reverse Transcriptase (Invitrogen).
Furthermore, analysis of sorted tetramer-positive cells from patient 476 demonstrated single fragment sizes for BV14 and BV17, which were identical to the fragment sizes generated from the selected clones, arguing for clonality of these dominant populations (Table 3).
The finding of a higher percentage of tetramer-positive CD8+ T-cells in tissues of macaque 819, infected intravenously, than in animal 817, infected intrarectally, may reflect a higher rate of viral replication in tissues of this animal at the time of sacrifice.
Clones confirmed to be tetramer-positive were expanded in T-25 flasks containing irradiated JY cells and fresh PBMCs in 25 ml of CTL medium containing PHA.
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