Example sentences for: template

How can you use “template” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Using the resulting plasmid pSB10 as template, the cloned product was PCR amplified with the vector forward and reverse primers, digested with Xba I and labeled as described above, resulting in Fragment B (see Figure 5).

  • The sequences and necessary concentrations in the PCR reaction are outlined in Table 2. The PCR mixture included 0.625 U AmpliTaq Gold DNA polymerase in the 2× SYBR Green Master Mix (PE Biosystems), the required concentration of specific forward and reverse primers, 10 ng of cDNA template and 0.25 U AmpErase UNG (PE Biosystems) in a 25 μl reaction volume.

  • Positive control reactions included template DNA isolated from human genomic phage that contained the appropriate sarcospan exons, and primers from the markers used to order the BAC map to ensure the quality of the DNA.

  • The Pearson correlation coefficient of the profile with the template was used as a simple measure of the agreement of the profile with the pattern.

  • The use of adaptamer sequences with identical T m 's for the 2 ndround of amplification optimized the yields, despite different product lengths and different template concentrations (which were dependent on the yield from the first round reactions).


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast