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Example sentences for: templates

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Polymerase activity of the HIV-1RT WT and insertion mutant enzymes was determined using two different template-primers: U-5PBS HIV-1 RNA and synthetic 49-mer U5-PBS DNA templates primed with the 17-mer PBS primer [ 36 ] . Assays were carried out in a 50 μL volume containing 50 mM Tris HCl, pH 7.8, 100 μg/mL bovine serum albumin, 5 mM MgCl 2 , 1 mM dithiothreitol, 60 mM KCl, 100 nM template-primer, 50 μM of each of the four dNTPs with one of them being 32P-labeled (0.
The source templates used were also prepared by standard PCR with the Stoffel fragment and the primer pair b1 and Jp on pBS (see figure 2).
The PCR labeling reaction was set up with 42 μl of DNA templates, 5 μl of 10x Clontech PCR buffer, 1 μl of 10 mM dNTPS containing 8 mM aminoallyl-dUTP and 2 mM dTTP, 1 μl of 10 μM CT primer or TT primer and 1 μl of Clontech cDNA polymerase.
Amplified products were diluted 10-fold in sterile water and 1 μl of diluted primary PCR products were added to 24 μl of secondary PCR master mix containing nested primers, 1 and 2R, to ensure specific amplification of double-stranded templates containing both adaptors.
Two other statistical tests were also performed to examine how information contained in conserved word-pair templates compared to other sequence features derived from S. cerevisiae , or from single conserved words.