Example sentences for: template-switching

How can you use “template-switching” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In brief, two rounds of amplification were carried out, which consisted of a combination of in vitro transcription and template-switching PCR.

  • The differences between several T7 based linear amplification protocols are mainly the following: 1. whether a template-switching mechanism is used in the synthesis of second strand cDNA, 2. what enzymes are used in the synthesis of second strand cDNA, 3. how ds cDNA is purified ("cleaned up") prior to in vitro transcription, and 4. how in vitro transcription is performed.

  • Template-switching does not affect the fidelity of amplification

  • [ 24 ] described a T7 based amplification protocol modified with a template-switching (TS) primer used to theoretically generate a full-length ds cDNA.


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