Words similar to tdt

Example sentences for: tdt

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After washing twice with 1.0 ml PBS, the cells were resuspended in 50 μl of TdT buffer containing 0.2 M potassium cacodylate, 2.5 mM Tris-HCL (pH 6.6), 2.5 mM CoCl 2 , 0.25 mg/ml bovine serum albumin, 5.0 units of terminal deoxynucleotidyl transferase and 0.5 nM biotinylated deoxyuridine triphosphate (b-UTP) and incubated at 37°C for 30 minutes.
The two-marker haplotypes D1S425-allele 10/D1S2827-allele 11, D1S490-allele 7/D1S227-allele 5, and D1S2616-allele 6/D1S549-allele 12 were significant in the TDT, C-TDT, and PDT.
Control cells were processed as described but without TdT.
ADPRT = adenosine diphosphate ribosyltransferase; cM = centiMorgan; C-TDT = combined S-TDT and TDT; ESRRG = estrogen-related receptor gamma; HLA = human leukocyte antigen; kb = kilobasepairs; LOD = logarithm of odds ratio; MIM = Mendelian Inheritance in Man (database; see [ 1]); PARP = poly ADP ribosyl polymerase; PCR = polymerase chain reaction; PDT = pedigree disequilibrium test; SLE = systemic lupus erythematosus; S-TDT = discordant-sib TDT; TDT = transmission disequilibrium test; T:NT = ratio of transmitted to nontransmitted alleles.
2 ; 0.06 mM 2-Mercaptoethanesulfonic Acid; 0.05 mg/mL BSA; 1 mM CoCl 2 ; 0.008 mM TdT dNTP mix; 300 U/mL TdT) and incubated for 60 minutes in a humidified chamber (37°C).