Words similar to tcpt-

Example sentences for: tcpt-

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The improved transposons, and <TcPT-1>, were created in the same way as , but Asc I sites were added to facilitate changing the fluorescent protein at a later date (supplemental material).
Insertion sites were identified for all PCR-positive transposed clones and all fluorescent /<TcPT-1> transposed clones by sequencing out of the transposon with a primer complimentary to the EGFP/ECFP coding region (5'-tggccgtttacgtcgccgtcca-3').
In addition, the two different reading frames present in the MEs were used to create the two different transposons, and ECFP was used in place of EGFP in <TcPT-1>.
Sequencing revealed 35 unique insertions (17 and 18 <TcPT-1>) and 10 repetitive insertions (figure 6A).
Independent transpositions of the GluR1 plasmid were performed with the EGFP and ECFP transposons ( and <TcPT-1>, respectively).