Words similar to tcpt-

Example sentences for: tcpt-

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Independent transpositions of the GluR1 plasmid were performed with the EGFP and ECFP transposons ( and <TcPT-1>, respectively).
Insertion sites were identified for all PCR-positive transposed clones and all fluorescent /<TcPT-1> transposed clones by sequencing out of the transposon with a primer complimentary to the EGFP/ECFP coding region (5'-tggccgtttacgtcgccgtcca-3').
Sequencing revealed 35 unique insertions (17 and 18 <TcPT-1>) and 10 repetitive insertions (figure 6A).
In addition, the two different reading frames present in the MEs were used to create the two different transposons, and ECFP was used in place of EGFP in <TcPT-1>.
The improved transposons, and <TcPT-1>, were created in the same way as , but Asc I sites were added to facilitate changing the fluorescent protein at a later date (supplemental material).