Words similar to tbst

Example sentences for: tbst

How can you use “tbst” in a sentence? Here are some example sentences to help you improve your vocabulary:

The membranes were blocked overnight at 4°C in TBST (10 mM Tris-HCl, 150 mM NaCl, 0.05% Tween; pH 8.0) containing 3% fat free dry milk.
The membranes were washed in Tris-buffered saline containing 0.05% Tween 20 (TBST), blocked by immersing the membrane in 0.5% casein in TBST for 1 h then incubated at 4°C overnight with affinity purified goat anti-ERβ N-19 antibody (Santa Cruz Biotechnology) diluted 1:4000 in blocking reagent.
Next, the nitrocellulose was incubated in TBST containing 2% non-fat dry milk containing primary antibody directed towards the indicated protein.
Thereafter, the membrane was incubated with a monoclonal anti-human EGF-R antibody (Oncogene Research Products, Cambridge, MA) diluted 1:10 in TBST at room temperature for 2 h.
Previous studies have demonstrated that these antibodies recognize the phosphorylated (active) forms of extracellular-regulated kinase (ERK), p38, and Jun-N-terminal kinase (JNK) Membranes were then washed (3 × 5 min each) with TBST buffer and incubated with anti-rabbit IgG horseradish peroxidase conjugate or anti-mouse IgG horseradish peroxidase conjugate (1:2000; Santa Cruz Biotechnology, Santa Cruz, CA) for 1 h at room temperature.