Words similar to tbst

Example sentences for: tbst

How can you use “tbst” in a sentence? Here are some example sentences to help you improve your vocabulary:

After washing in Tris-buffered saline with tween 20 (TBST), blots were incubated for one hour at room temperature with HRP-conjugated secondary antibody, washed again and detected by chemiluminescence.
Membranes were blocked in 5% nonfat dried milk for one hour, washed in 10 mM Tris-buffered saline (pH 7.5) containing 0.1% Tween-20 (TBST) and probed with primary antibody overnight at 4°C.
Thereafter, the membrane was incubated with a monoclonal anti-human EGF-R antibody (Oncogene Research Products, Cambridge, MA) diluted 1:10 in TBST at room temperature for 2 h.
Membranes were washed in TBST, incubated with the appropriate secondary antibody at 1/10000 dilution (Boehringer Mannheim) for 1 hr and protein was visualized by enhanced chemiluminescence (Amersham).
Following extensive washing in TBST, bands were visualized by the enhanced chemiluminescence method (Roche Molecular Biochemicals) and quantitated by laser densitometry.