Words similar to tbst
Example sentences for: tbst
How can you use “tbst” in a sentence? Here are some example sentences to help you improve your vocabulary:
The membranes were washed in Tris-buffered saline containing 0.05% Tween 20 (TBST), blocked by immersing the membrane in 0.5% casein in TBST for 1 h then incubated at 4°C overnight with affinity purified goat anti-ERβ N-19 antibody (Santa Cruz Biotechnology) diluted 1:4000 in blocking reagent.
Nonspecific binding was blocked with 5% fat free milk in TBST [50 mM Tris-HCL (pH7.
After incubation, the membranes were washed 1 × 15 min, 2 × 10 min and 4 × 5 min in fresh changes of TBST, and incubated for 1 hour in blocking buffer at room temperature.
TBST, Tris buffered saline Tween 20
After washing in TBST, the ERβ protein was visualized with a chemiluminescent detection system (ECL; Amersham Pharmacia Biotech, Piscataway, NJ) as recommended by the manufacturer, and exposed on Kodak XAR film (Eastman Kodak, Rochester, NY).