Words similar to tbst

Example sentences for: tbst

How can you use “tbst” in a sentence? Here are some example sentences to help you improve your vocabulary:

After blocking, the membranes were briefly rinsed in two changes of TBST, and washed once for 15 minutes and twice for 5 minutes in fresh changes of TBST.
Following three washes of 10 min each with TBST, the nitrocellulose was probed with horse-radish peroxidase-conjugated anti-IgG (Promega, Madison, WI) and developed using chemiluminescence detection (Pierce, Rockford, IL) according to the manufacturer's instructions.
Membranes were blocked in 5% BLOT-QuickBlocker PBST buffer (plus 0.1% Tween-20), washed three times with PBST buffer (plus 0.1% Tween-20), incubated in primary antibody diluted in femto/TBST buffer in a tray for 90 min at room temperature (or overnight at 4°C), washed five times (as above), incubated in secondary antibody-HRP (horseradish peroxidase)-conjugate (goat anti-rabbit) as above, washed four times with femto/TBST buffer followed by transfer to detection buffer and detection reagent.
After incubation, the membranes were washed 1 × 15 min, 2 × 10 min and 4 × 5 min in fresh changes of TBST, and incubated for 1 hour in blocking buffer at room temperature.
TBST buffer consisted of 50 mM Tris-HCl, 0.15 M NaCl, and 0.5% Tween 20, pH 8.0.