Words similar to tbst

Example sentences for: tbst

How can you use “tbst” in a sentence? Here are some example sentences to help you improve your vocabulary:

Membranes were washed in Tris-buffered saline containing 0.05% Tween 20 ( TBST ) and non-specific binding sites were blocked by immersing the membrane in blocking reagent (0.
TBST buffer consisted of 50 mM Tris-HCl, 0.15 M NaCl, and 0.5% Tween 20, pH 8.0.
After washing in Tris-buffered saline with tween 20 (TBST), blots were incubated for one hour at room temperature with HRP-conjugated secondary antibody, washed again and detected by chemiluminescence.
After washing in TBST, the filters were incubated with peroxidaseconjugated donkey anti-goat IgG secondary antibody (Jackson Immunoresearch) for 1 h at a dilution of 1:5000 in blocking reagent.
Next, the nitrocellulose was incubated in TBST containing 2% non-fat dry milk containing primary antibody directed towards the indicated protein.