Example sentences for: tbst

How can you use “tbst” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The membranes were blocked in TBST (10 mM Tris-HCl pH 8.0, 150 mM NaCl, 0.1% Tween-20) with 5% nonfat dry milk (w/v).

  • The membranes were blocked overnight at 4°C in TBST (10 mM Tris-HCl, 150 mM NaCl, 0.05% Tween; pH 8.0) containing 3% fat free dry milk.

  • After incubation, the membranes were washed 1 × 15 min, 2 × 10 min and 4 × 5 min in fresh changes of TBST, and incubated for 1 hour in blocking buffer at room temperature.

  • Membranes were washed with TBST and then incubated for 1 hour at room temperature with a secondary antibody followed by washing three times with TBST, immersed in ECL Plus western blotting detection system (Amersham Pharmacia Biotech, Buckinghamshire, England) for 1 min and then exposed to Kodak X-ray film (Rochester, NY).

  • After blocking, the membranes were briefly rinsed in two changes of TBST, and washed once for 15 minutes and twice for 5 minutes in fresh changes of TBST.


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