Words similar to tbst

Example sentences for: tbst

How can you use “tbst” in a sentence? Here are some example sentences to help you improve your vocabulary:

The filters were blocked with 2% nonfat dry milk in 10 mM Tris-HCl (pH 8.0), 0.15 M sodium chloride, 0.2% Tween-20 (TBST) then incubated with primary antibody (1:1000 dilution) for 2 hours at room temperature.
The membranes were washed 1 × 15 min, 2 × 10 min and 4 × 5 min in fresh changes of TBST, and incubated for 1 hour in blocking buffer at room temperature.
5% casein in TBST) for 1 hour at room temperature on an orbital shaker.
After washing in TBST, the filters were incubated with peroxidaseconjugated donkey anti-goat IgG secondary antibody (Jackson Immunoresearch) for 1 h at a dilution of 1:5000 in blocking reagent.
Previous studies have demonstrated that these antibodies recognize the phosphorylated (active) forms of extracellular-regulated kinase (ERK), p38, and Jun-N-terminal kinase (JNK) Membranes were then washed (3 × 5 min each) with TBST buffer and incubated with anti-rabbit IgG horseradish peroxidase conjugate or anti-mouse IgG horseradish peroxidase conjugate (1:2000; Santa Cruz Biotechnology, Santa Cruz, CA) for 1 h at room temperature.