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Example sentences for: tbst

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Previous studies have demonstrated that these antibodies recognize the phosphorylated (active) forms of extracellular-regulated kinase (ERK), p38, and Jun-N-terminal kinase (JNK) Membranes were then washed (3 × 5 min each) with TBST buffer and incubated with anti-rabbit IgG horseradish peroxidase conjugate or anti-mouse IgG horseradish peroxidase conjugate (1:2000; Santa Cruz Biotechnology, Santa Cruz, CA) for 1 h at room temperature.
Polyacrylamide gel electrophoresis and Western blot analyses were performed as described [ 13 23 ] . Briefly, samples were electrophoresed on 10% resolving sodium dodecyl sulphate-polyacrylamide gels (SDS/PAGE) according to the method of Laemmli [ 35 ] . Proteins were then electrotransferred onto nitrocellulose membranes and the membranes were incubated in TBST buffer containing 5% nonfat dry milk for 1 hour to block nonspecific protein binding.
Membranes were washed in Tris-buffered saline containing 0.05% Tween 20 ( TBST ) and non-specific binding sites were blocked by immersing the membrane in blocking reagent (0.
The membranes were washed in Tris-buffered saline containing 0.05% Tween 20 (TBST), blocked by immersing the membrane in 0.5% casein in TBST for 1 h then incubated at 4°C overnight with affinity purified goat anti-ERβ N-19 antibody (Santa Cruz Biotechnology) diluted 1:4000 in blocking reagent.
Nonspecific binding was blocked with 5% fat free milk in TBST [50 mM Tris-HCL (pH7.