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Example sentences for: tbz

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Next, we tested if the four other checkpoint radΔ alleles ( hus1Δ, rad1Δ, rad9Δ and rad17Δ ) conferred TBZ sensitivity (Figure 3).
Mutations that disrupt the microtubule cytoskeleton are also known to sensitize cells to TBZ [ 47 ] . However, using anti-tubulin immunofluorescence, we did not discover any gross microtubule cytoskeletal abnormalities in rad26Δ and rad3Δ cells (data not shown).
First, the Rad26-GFP dots were resistant to an extraction method that was developed to specifically-preserve the localization of chromatin-binding proteins [ 42 ] . Secondly, we discovered that the rad26Δ strain is sensitive to TBZ, a phenotype common to mutants that disrupt centromeric-heterochromatin [ 45 ] . Finally, fission yeast has a relatively small number of heterochromatic regions (3 centromeres, 6 telomeres, and 1 mating-type locus; [ 53 54 ] ) and the number of Rad26-GFP dots we observe is roughly consistent with localization to these regions.
Checkpoint rad mutants do not share TBZ Sensitivity
Therefore, TBZ sensitivity is caused by more than loss of Rad3-kinase activity alone.