Words similar to tbs-t

Example sentences for: tbs-t

How can you use “tbs-t” in a sentence? Here are some example sentences to help you improve your vocabulary:

Blots were blocked at room temperature for 1 hour in TBS-T (10 mM Tris, pH 8.0, 150 mM NaCl, 0.1% Tween-20) containing 5% dried fat-free milk, then incubated overnight at 4°C in TBS-T containing 0.5% milk and primary Ab (0.
1%; TBS-T) containing 4% chick egg ovalbulmin and 0.025% sodium azide.
The blots were washed (3 × 15 min) in TBS-T and specific labeling was visualized following enhanced chemiluminescence (Pierce, Rockford, IL) and exposure (10 seconds) to ECL-Hyperfilm (Amersham, Arlington Heights, IL).
The blots were washed (3 × 15 min) in TBS-T and were incubated for 2 hours with horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit, 1:5000, Pierce).
Blots were washed twice with TBS-T containing 0.5% milk, then incubated at room temperature for 2 hours in TBS-T containing 0.5% milk and 1:5000 dilutions of secondary Ab conjugated to peroxidase (for BMP-5, anti-goat IgG-peroxidase from Chemicon, Temecula CA; for Smad1, anti-rabbit Ig-peroxidase from Amersham, Piscataway, NJ).