Example sentences for: tbs-tween

How can you use “tbs-tween” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Samples were separated on 4-20% gradient Novex gels, transferred to Immobilon P membranes, blocked with 5% nonfat dry milk in TBS-Tween, then probed with anti-phospho-p44/42 MAPK antibody according to the manufacturer's recommendations.

  • 5 mM Tris, pH 6.7) at 50°C for 30 min, washed 4 times with TBS-Tween, blocked with 5% nonfat dry milk in TBS-Tween, then probed with anti-p44/42 ERK antibody according to the manufacturer's recommendations.

  • The membranes were then washed five times with TBS-Tween and were incubated with horseradish peroxidase-conjugated donkey anti-rabbit IgG (Bio-Rad, Hercules, CA), 1:5,000 dilution.

  • After three additional washes using TBS-Tween, the membrane was developed using ECL Western blotting system and Hyperfilm (Amersham Pharmacia Biotech, Piscataway, NJ).


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