Example sentences for: tbs

How can you use “tbs” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To visualize lipid membranes, sections were stained with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate ('DiI';DiIC 18 ; Molecular Probes) as previously described [ 24 ] . Sections were then washed several times with Tris buffered saline, and nucleic acids were stained by incubating sections in TBS containing 1 μM SYTOX Green (Molecular Probes) for 10 min at RT followed by several washes with TBS prior to confocal imaging.

  • Columns were washed with 10 column volumes of Wash Buffer (40 mM octylglucoside, 1 mM CaCl 2 , 1 mM MgCl 2 , TBS), before elution of bound protein with 10 mM EDTA in Wash Buffer.

  • This tissue was mounted in TBS tissue freezing medium (Triangle Biomedical Sciences, Durham, NC), frozen in isopentane cooled in liquid nitrogen, and stored at -80°C.

  • Cells were harvested by centrifugation (40-50 g typical yield), then washed once in 50 mM Tris, 150 mM NaCl, pH 7.5 (TBS).

  • Sections were washed and incubated for one hour with a 1:200 dilution of a secondary goat anti-rabbit Cy3-IgG antibody (Jackson Immuno-Research Laboratories, West Grove, PA), then washed in 1 × TBS for 2 × 10 min.


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