Words similar to tbs
Example sentences for: tbs
How can you use “tbs” in a sentence? Here are some example sentences to help you improve your vocabulary:
The cryo-sections were air-dried at room temperature, fixed in acetone for 5 min., washed for 15 min in Tris-buffered saline (TBS; 20 mM Tris-HCl, 500 mM NaCl, pH 7.4) and incubated for 1 hour with 1% purified milk protein blocking solution (Boehringer-Mannheim, Indianapolis, IN) in TBS to reduce background staining.
TBS, Tris-buffered saline
Cells were harvested by centrifugation (40-50 g typical yield), then washed once in 50 mM Tris, 150 mM NaCl, pH 7.5 (TBS).
The immunoprecipitates were pelleted, washed three times with wash buffer (1X TBS pH7.
Exponentially growing cells on slides were fixed in 4% paraformaldehyde in PEM buffer (80 mM Pipes [pH 7.0], 5 mM EGTA and 2 mM MgCl 2 ) for 30 min, permeabilized in 0.5% Triton X-100 in PEM buffer at room temperature for 15 min and rinsed three times with TBS + 0.1% Tween 20, followed by incubation in 0.5% nonfat dry milk in TBS + 0.1% Tween 20 for 2 h at room temperature.
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