Example sentences for: tbs

How can you use “tbs” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The cryo-sections were air-dried at room temperature, fixed in acetone for 5 min., washed for 15 min in Tris-buffered saline (TBS; 20 mM Tris-HCl, 500 mM NaCl, pH 7.4) and incubated for 1 hour with 1% purified milk protein blocking solution (Boehringer-Mannheim, Indianapolis, IN) in TBS to reduce background staining.

  • TBS, Tris-buffered saline

  • Cells were harvested by centrifugation (40-50 g typical yield), then washed once in 50 mM Tris, 150 mM NaCl, pH 7.5 (TBS).

  • The immunoprecipitates were pelleted, washed three times with wash buffer (1X TBS pH7.

  • Exponentially growing cells on slides were fixed in 4% paraformaldehyde in PEM buffer (80 mM Pipes [pH 7.0], 5 mM EGTA and 2 mM MgCl 2 ) for 30 min, permeabilized in 0.5% Triton X-100 in PEM buffer at room temperature for 15 min and rinsed three times with TBS + 0.1% Tween 20, followed by incubation in 0.5% nonfat dry milk in TBS + 0.1% Tween 20 for 2 h at room temperature.


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