Words similar to tar

Example sentences for: tar

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The TAR vector pARS-SV211-URA was constructed by insertion of the S. cerevisiae URA3 gene between the targeting hooks of pVC-SV211 [ 7 ] and cloning of ARSH4 into the polylinker.
ARS , autonomously replicating sequence; 5-FOA, 5-fluoroorotic acid; TAR, transformation-associated recombination; YAC, yeast artificial chromosome.
A low yield is predicted for a TAR vector containing two unique targeting hooks.
In Figure 2A, the TAR vector recombines with a genomic DNA fragment via the targeting hooks, which results in separation of the TATA box from the transcription initiation site in the URA3 gene by the DNA insert.
IN1R plus IN1L amply a 516-bp sequence in intron 1, and 46L plus 47R amplify a 575-bp sequence in exon 2, along with flanking introns [ 15 ] . We used a pair of primers specific to the zeta-globin promoter region to screen PCR transformants for the Tg.AC transgene sequence [ 7 ] . These primers generate a 419-bp PCR product that is diagnostic for recombination between the TAR vector and genomic Tg.AC transgene sequences.